| OT-674 suppresses photooxidative processes initiated by an RPE lipofuscin fluorophore. | |
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MedLine Citation:
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PMID: 18173705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pathological processes involved in age-related macular degeneration (AMD) include retinal pigment epithelial (RPE) cell degeneration; oxidative mechanisms likely contribute to the demise of these cells. Indeed, RPE cells may be particularly susceptible to photooxidative mechanisms since they accumulate retinoid-derived photoreactive compounds that constitute the lipofuscin of the cell. Thus we undertook to test the capacity of OT-674, the reduction product (Tempol-H) of the nitroxide Tempol, to suppress photooxidative processes initiated by the RPE lipofuscin fluorophore A2E. Accordingly, when ARPE-19 cells that had accumulated A2E were irradiated at 430 nm, pretreatment with OT-674 (0.01-10 mM) was found to confer a resistance to cell death. Monitoring by quantitative HPLC also showed that OT-674 reduced A2E photooxidation in a cell-free system. Moreover, when presented with a singlet oxygen generator, OT-674 served as a quencher of singlet oxygen that was more effective than Trolox and alpha-tocopherol. We conclude that OT-674 is a potent antioxidant that suppresses photooxidative processes generated in cultured RPE cells by the lipofuscin fluorophore A2E. As oxidative damage to RPE cells is considered to be a risk factor for AMD, antioxidant therapy with OT-674 may serve a protective role. |
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Authors:
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Jilin Zhou; Young P Jang; Stanley Chang; Janet R Sparrow |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 84 ISSN: 0031-8655 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2008 Jan-Feb |
Date Detail:
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Created Date: 2008-01-04 Completed Date: 2008-03-27 Revised Date: 2011-05-05 |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 75-80 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Columbia University, New York, NY, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
radiation effects Cell Line Cell-Free System Color Cyclic N-Oxides / pharmacology* Cytoprotection / drug effects Fluorescent Dyes / chemistry* Humans Hydroxylamine Light Lipofuscin / pharmacology* Oxidation-Reduction / drug effects Photochemistry Pigment Epithelium of Eye / cytology, drug effects*, radiation effects* Singlet Oxygen / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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EY 12951/EY/NEI NIH HHS; R01 EY012951-11/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cyclic N-Oxides; 0/Fluorescent Dyes; 0/Lipofuscin; 0/TEMPOL-H; 17778-80-2/Singlet Oxygen; 7803-49-8/Hydroxylamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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