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OC4 Influence of Seminal Plasma Added to Highly Diluted Semen on Sperm Motility of Young Boar Feed with Selenium and Vitamin E.
MedLine Citation:
PMID:  16984474     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
High dilution rates have been documented as detrimental for boar spermatozoa, shortening their lifespan (Centurion et al. 2003, Biol Reprod 69: 640-646). Addition of seminal plasma (SP) to semen extenders, or selenium (Se) and vitamin E (VE) in diet of boars could increase motility of highly diluted spermatozoa (HDS). The aim of this work was to evaluate the effect of seminal plasma on sperm motility of HDS from boars feed with Se and VE. Sixteen 12 month-old boars were designed to one of four dietary treatments: (i) control, Se 0 ppm-VE 0 IU/kg; (ii) Se 0-VE 250; (iii) Se 0.5-VE 250 and (iv) Se 0.5-VE 0. Boars were treated for 8 weeks before semen collection. Sperm rich fractions from each boar were diluted to 5 x 10(6) sperm/ml in PBS medium and incubated at 37 degrees C with or without 10% SP. The measurements were done at 0, 2 or 5 h. Data were analyzed as a mixed model for a factorial design [2 (Se) x 2 (VE) x 2 (SP) x 3 (h)]. Percentage of sperm motility (PSM) increases significantly (p < 0.001) with addition of Se (81.3 +/- 1.52), VE (81.0 +/- 1.62) and SP (81.5 +/- 1.57) vs control (73.4 +/- 1.61). There was significant interaction Se x VE (p < 0.001) and Se x VE x SP (p < 0.05) in PSM. However, PSM was affected significantly by time (0 h 83.4 +/- 1.92; 2 h 80.7 +/- 1.92 and 5 h 67.9 +/- 1.92; p < 0.001). There was significant interaction SP x Time (p < 0.05) in PSM. These results indicate that Se, VE and SP improve seminal viability. Addition of 10% of SP maintains PSM at least during 5 hours.
Authors:
F Centurión; S Echeverría; N Canul; R Ake; M Alfaro; R Santos; L Sarmiento
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  41 Suppl 2     ISSN:  0936-6768     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  103-4     Citation Subset:  IM    
Affiliation:
Faculty of Veterinary Medicine. Autonomous University of Yucatán, México.
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