Document Detail


O2-sensitive K+ current in undifferentiated and NGF-treated PC12 cell variants.
MedLine Citation:
PMID:  9172137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been reported that cultured PC12 cells can be used as a model for studying mechanisms of O2 sensitivity, previously examined in peripheral chemoreceptors and some neurons. This study compared the hypoxic depression of K+ currents in two PC12 variants, before and after differentiation into neurone-like cells induced by nerve growth factor (NGF). The results show that interaction of O2 and K+ channels is strongly-voltage dependent in the PC12/TM but not the PC12/ES subline. In PC12/TM cells an effect of hypoxia on the K+ current was appreciable only at moderately depolarized voltages, with a loss of sensitivity at +40 to +50 mV. NGF-induced transformation did not affect the responses seen in undifferentiated cells. These results emphasize the importance of screening PC12 cells before selecting a variant for studying O2 sensitivity. In view of evidence cited in the literature that hypoxia may effect membrane channels directly, further molecular and biophysical studies of the differences among PC12 variants are required.
Authors:
Y Kawai; J Win; A Comer; J Lipski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  8     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-08-07     Completed Date:  1997-08-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1369-74     Citation Subset:  IM    
Affiliation:
Department of Physiology, Faculty of Medicine and Health Science, University of Auckland, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Dopamine Antagonists / pharmacology*
Drug Evaluation, Preclinical
Genetic Variation*
Haloperidol / pharmacology*
Membrane Potentials / drug effects
Nerve Growth Factors / pharmacology*
Neurons / drug effects,  metabolism
Oxygen / pharmacology*
PC12 Cells
Patch-Clamp Techniques
Potassium Channels / drug effects*
Rats
Chemical
Reg. No./Substance:
0/Dopamine Antagonists; 0/Nerve Growth Factors; 0/Potassium Channels; 52-86-8/Haloperidol; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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