Document Detail

O-linked carbohydrates are required for FGF-2-mediated proliferation of mouse embryonic cells.
MedLine Citation:
PMID:  10610028     Owner:  NLM     Status:  MEDLINE    
During development, fibroblast growth factors (FGFs) serve highly specific functions that are mediated through high-affinity transmembrane receptors and modulated by membrane-bound proteoglycans. Proteoglycans, in an embryonic environment called embryoglycans, contain numerous carbohydrate ectodomains, the structure of which undergoes rearrangement. Since they can be lost from the cell surface, they are sometimes found in extracellular space where they may also serve some regulatory function. Here we address the potential roles of three naturally occurring isoforms of Lewis X (LeX) in FGF-2-mediated proliferation of embryonic stem (ES) cells. We have found that the addition of sulfated LeX to ES cells at a concentration of 17 nM promotes FGF-2 mitogenic activity while a 10-fold higher concentration leads to a reduction of FGF-2-mediated proliferation. Notably, this dose-dependent modulation operated only for sulfated LeX. Other fucosylated motifs, basic LeX trisaccharide and sialylated LeX, also affected ES cell proliferation but the mechanism cannot be clearly correlated with the presence or absence of FGF-2. The suppression of biosynthesis of O-linked carbohydrates including LeX reduced basal proliferation of ES cells and interfered with the mitogenic effect of FGF-2. However, in inhibitor-treated cells, the stimulatory activity of FGF-2 can be reestablished to its original level by exogenous LeX oligosaccharides. Our results show that (A) O-linked LeX oligosaccharides can regulate mitogenic activity of FGF-2 in embryonic cells, (B) and this ability varies with subtle modifications in their structure. Importantly, our data represent the first insight into the mechanism of how growth factor activities might be modulated by shedded embryoglycan ectodomains.
L Jirmanova; J Pacholikova; P Krejci; A Hampl; P Dvorak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  43     ISSN:  0214-6282     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  2000-01-31     Completed Date:  2000-01-31     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  555-62     Citation Subset:  IM    
Laboratory of Molecular Embryology, Mendel University Brno, Czech Republic.
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MeSH Terms
Antigens, CD15 / metabolism*
Cell Division / physiology
Cells, Cultured
Embryo, Mammalian / cytology,  metabolism*
Fibroblast Growth Factor 2 / metabolism*
Mice, Inbred Strains
Proteoglycans / biosynthesis,  metabolism*
Receptor Protein-Tyrosine Kinases / metabolism
Receptor, Fibroblast Growth Factor, Type 1
Receptor, Fibroblast Growth Factor, Type 2
Receptors, Fibroblast Growth Factor / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / metabolism
Uterus / metabolism
Reg. No./Substance:
0/Antigens, CD15; 0/Proteoglycans; 0/Receptors, Fibroblast Growth Factor; 103107-01-3/Fibroblast Growth Factor 2; EC protein, mouse; EC protein, mouse; EC Protein-Tyrosine Kinases; EC, Fibroblast Growth Factor, Type 1; EC, Fibroblast Growth Factor, Type 2

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