| O-acetylated sialic acids in gangliosides from pig spleen lymphocytes. | |
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MedLine Citation:
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PMID: 3235317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The sialic acid content of gangliosides from pig spleen lymphocytes was studied by thin-layer chromatography. N-glycolylneuraminic acid and N-acetylneuraminic acid were detected for the first time in this material as the major sialic acids. In addition, two other sialic acids, tentatively designated O-acetylated sialic acids, according to their RF values on cellulose plates, were also found. We have detected several gangliosides showing a retarded migration pattern in two dimensional thin-layer chromatography with an intermediate ammonia treatment. One of these gangliosides could be an O-acetylated derivative of the disialoganglioside GD3, since after de-O-acetyation it co-migrates with GD3. Another ganglioside co-migrated with GM2 before the alkaline treatment; however, after the treatment it was also retarded and co-migrates with GD3. |
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Authors:
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P Hueso; J A Cabezas; A Reglero |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Italian journal of biochemistry Volume: 37 ISSN: 0021-2938 ISO Abbreviation: Ital. J. Biochem. Publication Date: 1988 Sep-Oct |
Date Detail:
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Created Date: 1989-05-05 Completed Date: 1989-05-05 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376564 Medline TA: Ital J Biochem Country: ITALY |
Other Details:
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Languages: eng Pagination: 302-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, University of León, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Animals Chromatography, Thin Layer Gangliosides / analysis* Lymphocytes / analysis* Sialic Acids / analysis* Spleen / immunology* Swine |
| Chemical | |
Reg. No./Substance:
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0/Gangliosides; 0/Sialic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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