| O-GlcNAc protein modification in cancer cells increases in response to glucose deprivation through glycogen degradation. | |
| | |
MedLine Citation:
|
PMID: 19833729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
When cellular glucose concentrations fall below normal levels, in general the extent of protein O-GlcNAc modification (O-GlcNAcylation) decreases. However, recent reports demonstrated increased O-GlcNAcylation by glucose deprivation in HepG2 and Neuro-2a cells. Here, we report increased O-GlcNAcylation in non-small cell lung carcinoma A549 cells and various other cells in response to glucose deprivation. Although the level of O-GlcNAc transferase was unchanged, the enzyme contained less O-GlcNAc, and its activity was increased. Moreover, O-GlcNAcase activity was reduced. The studied cells contain glycogen, and we show that its degradation in response to glucose deprivation provides a source for UDP-GlcNAc required for increased O-GlcNAcylation under this condition. This required active glycogen phosphorylase and resulted in increased glutamine:fructose-6-phosphate amidotransferase, the first and rate-limiting enzyme in the hexosamine biosynthetic pathway. Interestingly, glucose deprivation reduced the amount of phosphofructokinase 1, a regulatory glycolytic enzyme, and blocked ATP synthesis. These findings suggest that glycogen is the source for increased O-GlcNAcylation but not for generating ATP in response to glucose deprivation and that this may be useful for cancer cells to survive. |
| | |
Authors:
|
Jeong Gu Kang; Sang Yoon Park; Suena Ji; Insook Jang; Sujin Park; Hyun Sil Kim; Sung-Min Kim; Jong In Yook; Yong-Il Park; Jürgen Roth; Jin Won Cho |
Related Documents
:
|
2283689 - Lactate does not enhance anoxia/reoxygenation damage in adult rat cardiac myocytes. 16242879 - Apts-labeled dextran ladder: a novel tool to characterize cell layer tightness. 8778219 - Characteristics of brin-bg5 and brin-bg7, two novel glucose-responsive insulin-secretin... 16663909 - Decline in energy reserves of chlorella sorokiniana upon exposure to ozone. 1336029 - Nutrient-induced activation of trehalase in nutrient-starved cells of the yeast sacchar... 17483239 - High-glucose-induced regulation of intracellular ang ii synthesis and nuclear redistrib... 1548759 - Detection of replication-competent and pseudotyped human immunodeficiency virus with a ... 1423239 - Partial characterization, progressive development and correlations of some neoplastic c... 18620249 - Cytometric investigations on hemocytes of the american oyster, crassostrea virginica. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-15 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 284 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-12-07 Completed Date: 2010-02-05 Revised Date: 2010-12-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 34777-84 Citation Subset: IM |
Affiliation:
|
Department of Biology, Oral Cancer Research Institute, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
AMP-Activated Protein Kinases
/
metabolism Acetylglucosamine / metabolism* Animals Cell Line Glucose / metabolism* Glycogen / metabolism* Humans N-Acetylglucosaminyltransferases / metabolism Neoplasms / metabolism* Protein Processing, Post-Translational* |
| Chemical | |
Reg. No./Substance:
|
50-99-7/Glucose; 7512-17-6/Acetylglucosamine; 9005-79-2/Glycogen; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC 2.4.1.-/O-GlcNAc transferase; EC 2.7.11.1/AMP-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pivotal role of the C-terminal DW-motif in mediating inhibition of pyruvate dehydrogenase kinase 2 b...
Next Document: Structural characterization of mouse neutrophil serine proteases and identification of their substra...