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Nutritional value of d: -amino acids, d: -peptides, and amino Acid derivatives in mice.
MedLine Citation:
PMID:  21956575     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This paper describes a method for determining the nutritional value of D: -amino acids, D: -peptides, and amino acid derivatives using a growth assay in mice fed a synthetic all-amino acid diet. A large number of experiments were carried out in which a molar equivalent of the test compound replaced a nutritionally essential amino acid such as L: -lysine (L: -Lys), L: -methionine (L: -Met), L: -phenylalanine (L: -Phe), and L: -tryptophan (L: -Trp) as well as the semi-essential amino acids L: -cysteine (L: -Cys) and L: -tyrosine (L: -Tyr). The results show wide-ranging variations in the biological utilization of test substances. The method is generally applicable to the determination of the biological utilization and safety of any amino acid derivative as a potential nutritional source of the corresponding L: -amino acid. Because the organism is forced to use the D: -amino acid or amino acid derivative as the sole source of the essential or semi-essential amino acid being replaced, and because a free amino acid diet allows better control of composition, the use of all-amino acid for such determinations may be preferable to protein-based diets.
Authors:
Mendel Friedman; Carol E Levin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  794     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-09-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  337-53     Citation Subset:  IM    
Affiliation:
United States Department of Agriculture, Western Regional Research Center, Agricultural Research Service, Albany, CA, USA, mendel.friedman@ars.usda.gov.
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