Document Detail


Nutritional requirements of two flower spiroplasmas and honeybee spiroplasma.
MedLine Citation:
PMID:  6848486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A chemically defined medium (CC-494) was used to study the nutritional requirements of three spiroplasmas representing three distinct serogroups: flower spiroplasmas [Spiroplasma floricola and FS (SR-3)] and honeybee spiroplasma [HBS (AS-576)]. Glucose, fructose, and mannose were utilized by all three spiroplasmas. In addition, the honeybee spiroplasma could ferment trehalose, FS (SR-3) could ferment sucrose, and S. floricola could ferment trehalose, sucrose, and raffinose. The three spiroplasmas varied greatly in their requirements of amino acids for growth. S. floricola was the only strain that utilized arginine. HBS (AS-576) required at least one purine and one pyrimidine base (either free base or ribonucleoside) for growth, while both flower spiroplasmas grew with only one base in the medium. Oleic acid, cholesterol, and bovine serum albumin were essential to all three spiroplasmas. Palmitic acid, which was nonessential, promoted growth significantly.
Authors:
C J Chang; T A Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  153     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-02-25     Completed Date:  1983-02-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  452-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Arginine / metabolism
Bees / microbiology
Carbohydrate Metabolism*
Cholesterol / pharmacology
Culture Media
Fatty Acids / pharmacology
Plants / microbiology
Purines / pharmacology
Pyrimidines / pharmacology
Ribonucleosides / pharmacology
Serum Albumin, Bovine / pharmacology
Spiroplasma / physiology*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Fatty Acids; 0/Purines; 0/Pyrimidines; 0/Ribonucleosides; 0/Serum Albumin, Bovine; 57-88-5/Cholesterol; 74-79-3/Arginine
Comments/Corrections

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