| Nutritional requirements for synthesis of heat-stable enterotoxin by Yersinia enterocolitica. | |
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MedLine Citation:
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PMID: 8250556 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A defined medium that supported the growth of and synthesis of heat-stable enterotoxin (YST) by clinical isolates of Yersinia enterocolitica at levels equivalent to those observed in a complex Trypticase soy broth-0.6% yeast extract medium was developed. The defined medium contained four amino acids (L-methionine, L-glutamic acid, glycine, and L-histidine), inorganic salts, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) buffer, and potassium gluconate as the carbon source. Methionine was required for growth by most strains of Y. enterocolitica used in this study; thus, it was not possible to determine whether it was also required for the synthesis of YST. The other 17 amino acids commonly found in proteins did not stimulate the synthesis of YST when added to the defined medium. The yield of YST observed with other carbon sources fermented by Y. enterocolitica ranged from 4- to 26-fold lower than that obtained with potassium gluconate. The divalent cations Ca2+ and Mn2+ had no effect on the synthesis of YST; however, concentrations of Fe2+ above 10 microM inhibited the synthesis of the enterotoxin. The addition of a mixture of pyrimidines containing thymine, cytosine, and uracil, each at a concentration of 2.0 mM, stimulated the synthesis of YST by 10 to 15%, whereas a mixture of adenine and guanine, each at a similar concentration, inhibited the synthesis of YST. Vitamins had no effect on the amounts of YST produced by Y. enterocolitica strains grown in the defined medium.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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N Amirmozafari; D C Robertson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 59 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1994-01-05 Completed Date: 1994-01-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3314-20 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Kansas, Lawrence 66045. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Bacterial Toxins / biosynthesis* Culture Media Enterotoxins / biosynthesis* Humans Virulence Yersinia enterocolitica / growth & development, metabolism*, pathogenicity |
| Grant Support | |
ID/Acronym/Agency:
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AI-12357/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Bacterial Toxins; 0/Culture Media; 0/Enterotoxins; 0/heat-stable enterotoxin (Yersinia) |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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