Document Detail

Nutritional requirements of mycelial growth of Cordyceps sinensis in submerged culture.
MedLine Citation:
PMID:  16108789     Owner:  NLM     Status:  MEDLINE    
AIMS: The nutritional requirements for mycelial growth of Cordyceps sinensis in semi-synthetic liquid media were investigated. The results provide a basis for further physiological study and industrial fermentation of the fungus. METHODS AND RESULTS: Nutritional requirements, including 17 carbohydrates, 16 nitrogen compounds, nine vitamins, four macro-elements, four trace-elements and eight ratios of carbon to nitrogen, were studied for their effects on the mycelial growth in submerged cultures of C. sinensis by using one-factor-at-a-time and orthogonal matrix methods. Among these variables, sucrose, peptone, folic acid, calcium, zinc and a carbon to nitrogen ratio 12 : 1 were identified as the requirements for the optimum mycelial growth. The concentrations of sucrose, peptone and yeast extract were optimized and the effects of medium composition on mycelial growth were found to be in the order sucrose > yeast extract > peptone. The optimal concentration for mycelial growth was determined as 50 g l(-1) sucrose, 10 g l(-1) peptone and 3 g l(-1) yeast extract. CONCLUSIONS: Under optimal culture conditions, over 22 g l(-1) of mycelial biomass could be obtained after 40 days in submerged cultures. SIGNIFICANCE AND IMPACT OF THE STUDY: Cordyceps sinensis, one of the most valued medicinal fungi, is shown to grow in axenic culture. This is the first report on nutritional requirements and design of a simplified semi-synthetic medium for mycelial growth of this psychrophilic species, which grows slowly below 20 degrees C. The results of this study will facilitate research on mass production of the fungus under defined culture conditions.
C-H Dong; Y-J Yao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  99     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-08-19     Completed Date:  2006-02-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  483-92     Citation Subset:  IM    
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
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MeSH Terms
Carbohydrates / physiology
Carbon / physiology
Cordyceps / growth & development*
Culture Media
Mycelium / growth & development*
Nitrogen / physiology
Nitrogen Compounds / metabolism
Nutritional Physiological Phenomena / physiology*
Peptones / metabolism
Sucrose / metabolism
Vitamins / physiology
Yeasts / metabolism
Reg. No./Substance:
0/Carbohydrates; 0/Culture Media; 0/Elements; 0/Nitrogen Compounds; 0/Peptones; 0/Vitamins; 57-50-1/Sucrose; 7440-44-0/Carbon; 7727-37-9/Nitrogen

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