Document Detail


Nutritional Effects on the Growth, Cell Size, and Resistance to Stress of Acholeplasma laidlawii.
MedLine Citation:
PMID:  21502068     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Due to their lack of a cell wall, mycoplasmas are useful models for the study of biological membranes. However, they are much less appreciated by cell culture laboratories due to their unfortunate tendency to contaminate cell culture lines (1-5). One of the primary protections of a cell line is through filtration of cell culture media. It is necessary to use 0.1 μm-rated filters because mycoplasmas can often penetrate the more commonly used 0.2 (or 0.22) μm-rated filters. In the battle against mycoplasma contamination, it is helpful to delineate factors that may affect mycoplasma replication, cell size, and resistance to contamination control. The addition of cholesterol and unsaturated long chain fatty acids to growth media enhance replication of Acholeplasma laidlawii (6-10). The absence of glucose, presence of unsaturated fatty acids, increased incubation period, and exposure to stress promote the production of small mycoplasma cells (6-12). Exposure to a high salt concentration, hydrogen peroxide, and UV light, which are commonly used for contamination control, can enhance the production of small resistant cells (13-15).
Authors:
Martha Folmsbee; Courtney Noah; Morven McAlister
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  PDA journal of pharmaceutical science and technology / PDA     Volume:  64     ISSN:  1079-7440     ISO Abbreviation:  PDA J Pharm Sci Technol     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2011-04-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9439538     Medline TA:  PDA J Pharm Sci Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  581-92     Citation Subset:  IM    
Affiliation:
Pall Corporation.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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