| Nutrient profiling reveals potent inducers of trichothecene biosynthesis in Fusarium graminearum. | |
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MedLine Citation:
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PMID: 19406250 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fusarium head blight is one of the most important diseases of wheat worldwide due to crop losses and the contamination of grains with trichothecene mycotoxins. The biosynthesis of trichothecenes by Fusarium spp. is highest during infection, but relatively low levels are produced from saprophytic growth in axenic culture. A strain of Fusarium graminearum was constructed where the promoter from the TRI5 trichothecene biosynthesis gene was fused to GFP. Using this strain in large-scale nutrient profiling, a variety of amines were identified that significantly induce TRI5 expression. Analysis of trichothecene levels in the culture filtrates revealed accumulation of the toxin to over 1000ppm in response to these inducers, levels either greater than or equivalent to those observed during infection. From this work, we propose that products of the arginine-polyamine biosynthetic pathway in plants may play a role in the induction of trichothecene biosynthesis during infection. |
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Authors:
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Donald M Gardiner; Kemal Kazan; John M Manners |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-03 |
Journal Detail:
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Title: Fungal genetics and biology : FG & B Volume: 46 ISSN: 1096-0937 ISO Abbreviation: Fungal Genet. Biol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-06-17 Completed Date: 2009-08-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607601 Medline TA: Fungal Genet Biol Country: United States |
Other Details:
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Languages: eng Pagination: 604-13 Citation Subset: IM |
Affiliation:
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CSIRO Plant Industry, Queensland Bioscience Precinct, Queensland, Australia. donald.gardiner@csiro.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Artificial Gene Fusion Culture Media / chemistry* Fusarium / metabolism* Gene Expression Profiling Genes, Reporter Green Fluorescent Proteins / genetics, metabolism Trichothecenes / biosynthesis* Triticum / microbiology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Trichothecenes; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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