| Nutrient and environmental growth factors for nine oral small-sized spirochete strains containing one endoflagellum from each cell end. | |
| | |
MedLine Citation:
|
PMID: 2719849 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The present investigation was carried out in order to obtain better information about the growth requirements of small-sized spirochetes containing one endoflagellum from each cell end. Nine strains of such spirochetes were isolated from subgingival plaque in patients suffering from advanced marginal periodontitis. The strains were maintained in fluid NOS medium with 0.07% Noble Agar. The following environmental factors were studied: Oxygen in the incubation atmosphere and the pH value of the medium. The following nutrient factors were studied: Rabbit serum, heart infusion and trypticase, bovine albumin, human globulin, long-chain fatty acids, volatile fatty acids, steroids, amino acids, carbohydrates, and Na-bicarbonate. Growth was normally determined after 3 days' incubation at 35 degrees C by counting numbers of spirochete cells in a Petroff-Hausser counting chamber. All strains tolerated 3% oxygen in the atmosphere and the pH-optimum was 7.5. Rabbit serum was found not to be an essential nutrient factor. Human globulin stimulated growth, while bovine albumin inhibited growth. Heart infusion and trypticase influenced growth moderately. Of the remaining nutrient factors the long-chain fatty acids inhibited growth and the volatile fatty acids did not affect growth, while certain carbohydrates, especially glucose, stimulated growth. Na-bicarbonate in low concentrations stimulated growth, while higher concentrations inhibited growth. |
| | |
Authors:
|
N E Fiehn |
Related Documents
:
|
2863739 - The influence of incubation time and medium composition on fatty acid production by som... 20572249 - Streptococcus mutans inhibits candida albicans hyphal formation by the fatty acid signa... 15002669 - Levels and immunolocalization of endogenous cytokinins in thidiazuron-induced shoot org... 16661939 - Azido auxins: synthesis and biological activity of fluorescent photoaffinity labeling a... 6695039 - An acidic extracellular environment reduces the fixation of radiation damage. 10679049 - The effect of a methanogen, methanobrevibacter smithii, on the growth rate, organic aci... 2181659 - Vertical distribution and flows of lead and natural radionuclides in the atmosphere. 21250819 - Synthesis, hydrolysis studies and phamacodynamic profiles of amide prodrugs of dexibupr... 4030759 - Metabolism of all-trans-retinoic acid and all-trans-retinyl acetate. demonstration of c... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 97 ISSN: 0903-4641 ISO Abbreviation: APMIS Publication Date: 1989 Apr |
Date Detail:
|
Created Date: 1989-06-28 Completed Date: 1989-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8803400 Medline TA: APMIS Country: DENMARK |
Other Details:
|
Languages: eng Pagination: 287-96 Citation Subset: IM |
Affiliation:
|
Department of Microbiology, Royal Dental College, Copenhagen, Denmark. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood / metabolism Carbohydrate Metabolism Culture Media Dental Plaque / microbiology Flagella Humans Hydrogen-Ion Concentration Oxygen Consumption Periodontitis / microbiology Proteins / metabolism Rabbits Spirochaetales / growth & development*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Review of states' practices on the use of psychotropic medication.
Next Document: Mucosal cysts in Barrett's mucosa with dysplasia.