Document Detail


Nurse cell-oocyte interaction: a new F-actin mesh associated with the microtubule-rich core of an insect ovariole.
MedLine Citation:
PMID:  18620180     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Fluorescent staining of Rhodnius prolixus ovarioles with rhodaminylphalloidin revealed an elaborate interconnecting meshwork of F-actin encasing the microtubule rich core. The thick actin struts have branches extending partially into the nurse cell lobes extending from the syncytical core. Basally the network ends at the nurse cell-pre-follicular interface and does not extend into the trophic cords. Light microscopy, TEM and SEM show the dense fibrous struts have numerous branch points creating a porous cylindrical collar at the core periphery. The filaments are organized in a dense felt-like anisotropic meshwork rather in parallel arrays. This morphology coupled with the stability of the entire structure in extraction procedures suggests the actin filaments are crosslinked, presumably by associated proteins. Detergent extraction experiments indicate isolation of the mesh is possible. Comparison of Nomarski DIC images with polarizing microscopy images of the same preparations show the F-actin struts are not birefringent while the microtubules of the core are highly birefringent and resistant to nocodazole. Indirect immunocytochemical staining of sections for actin confirmed the F-actin distribution visualized with phalloidin and contrasted markedly with staining pattern for tubulin. The discovery of this prominent actin meshwork must now be incorporated into models attempting to explain the mechanisms underlying polarized nurse cell-oocyte transport.
Authors:
E Huebner; H Gutzeit
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tissue & cell     Volume:  18     ISSN:  0040-8166     ISO Abbreviation:  Tissue Cell     Publication Date:  1986  
Date Detail:
Created Date:  2008-07-14     Completed Date:  2009-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0214745     Medline TA:  Tissue Cell     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  753-64     Citation Subset:  -    
Affiliation:
Department of Zoology, University of Manitoba, Manitoba, Canada.
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