Document Detail

Numerous adrenal chromaffin cell preparations fail to produce analgesic effects in the formalin test or in tests of acute pain even with nicotine stimulation.
MedLine Citation:
PMID:  11050373     Owner:  NLM     Status:  MEDLINE    
Previous studies have reported that intrathecal implants of a variety of adrenal chromaffin cell preparations all produce analgesic effects in rodents. The major objective of the present study was to determine if any adrenal chromaffin cell preparations produce more robust analgesic effects than other cell preparations. The present study included adult rat adrenal chromaffin tissue allografts, purified adult bovine chromaffin cells, and polymer-encapsulated calf adrenal chromaffin cells, all prepared according to previously published procedures, as well as purified calf adrenal chromaffin cells. Previous studies have also suggested that immunosuppression may play a role in graft survival, and potentially increase the magnitude of analgesic effects, so the present study included both immunosuppressed and non-immunosuppressed groups (cyclosporin A, 10 mg/kg per day). Behavioral tests included the formalin test; and a dorsal tail-flick, hot-plate, and paw-pinch test, conducted sequentially 2 min after systemic nicotine (0.1 mg/kg) to evoke release from the chromaffin cells, as previously reported. Analgesic effects related to morphine and nicotine were detected, and consistent differences in performance could be detected between individual animals. Surprisingly, no analgesic effects were detectable with any of the four chromaffin cell preparations, with or without immunosuppression, in the formalin test or with nicotine stimulation in tests of acute pain.
M D Lindner; J M Francis; P E McDermott; W J Bell; T J Blaney; S S Sherman; J A Saydoff
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pain     Volume:  88     ISSN:  0304-3959     ISO Abbreviation:  Pain     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-01-08     Completed Date:  2001-01-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7508686     Medline TA:  Pain     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  177-88     Citation Subset:  IM    
CytoTherapeutics, Inc., Two Richmond Square, Providence, RI 02906, USA.
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MeSH Terms
Acute Disease
Adrenal Glands / cytology,  physiology*
Behavior, Animal / drug effects
Cell Transplantation / physiology*
Chromaffin Cells / physiology*
Cyclosporine / pharmacology
Formaldehyde / diagnostic use
Immunosuppressive Agents / pharmacology
Nicotine / pharmacology*
Nicotinic Agonists / pharmacology*
Pain Measurement / drug effects*
Rats, Sprague-Dawley
Stimulation, Chemical
Transplantation, Heterologous
Reg. No./Substance:
0/Immunosuppressive Agents; 0/Nicotinic Agonists; 50-00-0/Formaldehyde; 54-11-5/Nicotine; 59865-13-3/Cyclosporine
Comment In:
Pain. 2002 Jan;95(1-2):191-4   [PMID:  11790482 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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