| Nucleus pulposus cell response to confined and unconfined compression implicates mechanoregulation by fluid shear stress. | |
| | |
MedLine Citation:
|
PMID: 21132369 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Mechanical loading of the intervertebral disc (IVD) plays an important role in governing the function of nucleus pulposus (NP) cells. In situ, the loading environment of the NP is neither fully confined nor unconfined. To investigate the potential influences of these idealized loading modes, we utilized an alginate cell culture system to compare the effects of 1 h confined and unconfined compression on glycoprotein gene expression in mixed population NP cells. For dynamic compression, we applied 10 kPa peak stress using a sinusoidal waveform at 0.5 Hz. For static compression, displacements were applied at a constant rate and held at 20% strain. Aggrecan, laminin, fibronectin, glypican, biglycan, and fibromodulin were generally stimulated, and trends were similar between confined and unconfined compression. On the other hand, lumican expression was differentially regulated between confined and unconfined groups. More interestingly, trends under static loading were opposite that under dynamic loading; lumican was upregulated in static confined compression and in dynamic unconfined compression. We theorize that this pattern is indicative of regulation by fluid flow within loaded alginate disks. Calculations using the linear biphasic model suggest that spatial pressure gradients exist in static confined specimens for the majority of the loading duration. Using a parallel plate flow chamber, we report for the first time that NP cells are sensitive to fluid shear stress and confirm that lumican expression can be regulated by short durations of exposure to fluid shear. |
| | |
Authors:
|
Ping Wang; Li Yang; Adam H Hsieh |
Related Documents
:
|
22325349 - Peroxiredoxins as molecular triage agents, sacrificing themselves to enhance cell survi... 16478759 - Cytotrophoblast stem cell lines derived from human embryonic stem cells and their capac... 2849599 - Selected human t cell lines respond to thymopoietin with intracellular cyclic gmp eleva... 18397749 - International banking: checks, deposits, and withdrawals. 1161259 - Studies of tumor cell lines derived from patients with malignant melanoma. 18726349 - Cloning and characterization of a cdna encoding human differentiation antigen 5d4. 23245859 - In vitro cytotoxicity of zoledronate (nitrogen-containing bisphosphonate: nbp) and/or e... 21748689 - Characterizing system performance in total internal reflection fluorescence microscopy. 12216959 - Potential uses of milk epithelial cells: a review. |
Publication Detail:
|
Type: Journal Article Date: 2010-12-04 |
Journal Detail:
|
Title: Annals of biomedical engineering Volume: 39 ISSN: 1521-6047 ISO Abbreviation: Ann Biomed Eng Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-02-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0361512 Medline TA: Ann Biomed Eng Country: United States |
Other Details:
|
Languages: eng Pagination: 1101-11 Citation Subset: IM |
Affiliation:
|
Fischell Department of Bioengineering, University of Maryland, Jeong H. Kim Engineering Building, Room 3242, College Park, MD, 20742, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Computational model of the human elbow and forearm: application to complex varus instability.
Next Document: Multifunctional FePt Nanoparticles for Radiation-Guided Targeting and Imaging of Cancer.