Document Detail

Nucleus pulposus cell response to confined and unconfined compression implicates mechanoregulation by fluid shear stress.
MedLine Citation:
PMID:  21132369     Owner:  NLM     Status:  MEDLINE    
Mechanical loading of the intervertebral disc (IVD) plays an important role in governing the function of nucleus pulposus (NP) cells. In situ, the loading environment of the NP is neither fully confined nor unconfined. To investigate the potential influences of these idealized loading modes, we utilized an alginate cell culture system to compare the effects of 1 h confined and unconfined compression on glycoprotein gene expression in mixed population NP cells. For dynamic compression, we applied 10 kPa peak stress using a sinusoidal waveform at 0.5 Hz. For static compression, displacements were applied at a constant rate and held at 20% strain. Aggrecan, laminin, fibronectin, glypican, biglycan, and fibromodulin were generally stimulated, and trends were similar between confined and unconfined compression. On the other hand, lumican expression was differentially regulated between confined and unconfined groups. More interestingly, trends under static loading were opposite that under dynamic loading; lumican was upregulated in static confined compression and in dynamic unconfined compression. We theorize that this pattern is indicative of regulation by fluid flow within loaded alginate disks. Calculations using the linear biphasic model suggest that spatial pressure gradients exist in static confined specimens for the majority of the loading duration. Using a parallel plate flow chamber, we report for the first time that NP cells are sensitive to fluid shear stress and confirm that lumican expression can be regulated by short durations of exposure to fluid shear.
Ping Wang; Li Yang; Adam H Hsieh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-12-04
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  39     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-10     Completed Date:  2011-06-02     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1101-11     Citation Subset:  IM    
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
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MeSH Terms
Body Fluids / physiology*
Cells, Cultured
Compressive Strength / physiology
Computer Simulation
Feedback, Physiological / physiology
Intervertebral Disc / cytology*,  physiology*
Mechanotransduction, Cellular / physiology*
Models, Biological*
Rats, Sprague-Dawley
Shear Strength / physiology
Stress, Mechanical
Weight-Bearing / physiology*
Grant Support

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