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Nucleotide sequence of the transcriptional repressor gene korB which plays a key role in regulation of the copy number of broad host range plasmid RK2.
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MedLine Citation:
PMID:  3309894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The product of the korB gene of broad host range plasmid RK2 is one of at least two proteins which repress transcription of the essential replication gene trfA. We report here the nucleotide sequence of korB and the properties of its predicted polypeptide product KorB which has a molecular weight of 39,011 Da. KorB is likely to be a soluble protein with an overall net negative charge. However, consistent with a role in transcriptional regulation, there is a region with extensive homology to the alpha helix-turn-alpha helix motif of many DNA binding proteins. This region shows no significant homology to equivalent regions of the TrfB protein which is the primary transcriptional repressor of RK2 and which binds to an operator whose half sites show considerable homology to the half sites of the korB operator.
Authors:
B D Theophilus; C M Thomas
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  15     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1987 Sep 
Date Detail:
Created Date:  1987-11-18     Completed Date:  1987-11-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  7443-50     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Birmingham, UK.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Y00448
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bacterial Proteins / genetics*
Base Sequence
DNA-Binding Proteins / genetics
Escherichia coli / genetics*
Genes
Genes, Bacterial*
Molecular Sequence Data
Plasmids*
Sequence Homology, Nucleic Acid
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/KorB protein, Plasmid RK2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 9 Year: 1987
Volume: 15 Issue: 18
First Page: 7443 Last Page: 7450
ID: 306259
PubMed Id: 3309894

Nucleotide sequence of the transcriptional repressor gene korB which plays a key role in regulation of the copy number of broad host range plasmid RK2.
B D Theophilus
C M Thomas
Department of Genetics, University of Birmingham, UK.



Article Categories:
  • Research Article


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