Document Detail


Nucleotide sequence of the lysozyme gene of bacteriophage T4. Analysis of mutations involving repeated sequences.
MedLine Citation:
PMID:  6302287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nucleotide sequence of the lysozyme (e) gene of bacteriophage T4 and approximately 130 additional nucleotides on each side has been determined. The 5'-end of the gene for internal protein III appears to be located about 70 base-pairs from the 3'-end of the lysozyme gene. Nucleotide sequence analysis of mutant e genes confirmed that three identified hotspots of frameshift mutations are runs of five A nucleotides in the wild-type gene. The endpoints of two deletions are direct repeats of eight base-pairs in the wild-type gene. Two frameshift mutations with high reversion frequencies are duplications of five or seven base-pairs. The cloning and nucleotide sequence determination of the lysozyme gene will facilitate further study of the molecular biology of T4 lysozyme.
Authors:
J E Owen; D W Schultz; A Taylor; G R Smith
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of molecular biology     Volume:  165     ISSN:  0022-2836     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-06-23     Completed Date:  1983-06-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  229-48     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/J02514;  V00860
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Codon
DNA Restriction Enzymes
DNA, Viral
Electrophoresis, Polyacrylamide Gel
Genes, Viral*
Muramidase / genetics*
Mutation
Repetitive Sequences, Nucleic Acid
T-Phages / enzymology,  genetics*
Viral Proteins / genetics
Grant Support
ID/Acronym/Agency:
AI13079/AI/NIAID NIH HHS; GM22731-04/GM/NIGMS NIH HHS; GM26798/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Codon; 0/DNA, Viral; 0/Viral Proteins; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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