Document Detail


Nucleotide sequence and expression of a cloned Thiobacillus ferrooxidans recA gene in Escherichia coli.
MedLine Citation:
PMID:  2504646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nucleotide sequence of the recA gene of Thiobacillus ferrooxidans has been determined. No SOS box characteristic of LexA-regulated promoters could be identified in the 196-bp region upstream from the coding region. The cloned T. ferrooxidans recA gene was expressed in Escherichia coli from both the lambda pR and lac promoters. It was not expressed from the 2.2-kb of T. ferrooxidans DNA preceding the gene. The T. ferrooxidans recA gene specifies a protein of 346 amino acids that has 66% and 69% homology to the RecA proteins of E. coli and Pseudomonas aeruginosa, respectively. Most amino acids that have been identified as being of functional importance in the E. coli RecA protein are conserved in the T. ferrooxidans RecA protein. Although some amino acids that have been associated with proteolytic activity have been substituted, the cloned protein has retained protease activity towards the lambda and E. coli LexA repressors.
Authors:
R S Ramesar; V Abratt; D R Woods; D E Rawlings
Related Documents :
8807806 - Transcription of the campylobacter jejuni cell division gene ftsa.
8675016 - Cloning, sequencing and expression of the uvra gene from an extremely thermophilic bact...
3012466 - Acinetobacter calcoaceticus encoded mutarotase: nucleotide sequence analysis of the gen...
9043126 - 2-phenylethylamine catabolism by escherichia coli k-12: gene organization and expression.
7828066 - On an efficient parallelization of exhaustive sequence comparison algorithms on message...
19904236 - Characterization of genome integrity for oversized recombinant aav vector.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Gene     Volume:  78     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-10-10     Completed Date:  1989-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M26933
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Blotting, Western
Cloning, Molecular
DNA, Bacterial / genetics
Escherichia coli / genetics
Gene Expression Regulation*
Molecular Sequence Data
Peptide Hydrolases
Pseudomonas aeruginosa / genetics
Rec A Recombinases / genetics*
Thiobacillus / genetics*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; EC 2.7.7.-/Rec A Recombinases; EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Mutant alleles of the meiotic locus, mei-9, differ in degree of effects on rod chromosome magnificat...
Next Document:  Identification and characterization of the nifH and nifJ promoter regions located on the nif-plasmid...