| Nucleotide sequence analysis of the unusually long terminal inverted repeats of a giant linear plasmid, SCP1. | |
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MedLine Citation:
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PMID: 1749818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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SCP1 is a giant linear plasmid of 350 kb coding for the methylenomycin biosynthetic genes in Streptomyces coelicolor. The unusually long terminal inverted repeats present on both ends of SCP1 were analyzed on the nucleotide sequence level. Analysis of six clones containing the terminal 0.35-kb XbaI fragment revealed a slight heterogeneity in the nucleotide sequences of the SCP1 ends. Moreover, it was indicated that this fragment contained seven palindromic inverted repeats and a GT-rich region in the 5'-end strand. The size of the terminal inverted repeats was determined to be 81 kb by the cloning and sequencing of their end-points. An insertion sequence, IS466 was shown to be present just at the end of the right terminal inverted repeat. |
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Authors:
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H Kinashi; M Shimaji-Murayama; T Hanafusa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plasmid Volume: 26 ISSN: 0147-619X ISO Abbreviation: Plasmid Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1992-01-21 Completed Date: 1992-01-21 Revised Date: 2006-05-01 |
Medline Journal Info:
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Nlm Unique ID: 7802221 Medline TA: Plasmid Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 123-30 Citation Subset: IM |
Affiliation:
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Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/S70700; S70701; S70702; S70703; X55687; X55688; X55689; X55690; X56856; X58180 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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biosynthesis* Base Sequence DNA, Bacterial / genetics Electrophoresis Molecular Sequence Data Peptides Plasmids / genetics* Repetitive Sequences, Nucleic Acid* Restriction Mapping Streptomyces / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/DNA, Bacterial; 0/Peptides; 52775-76-5/methylenomycin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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