Document Detail

Nucleotide sequence analysis of the unusually long terminal inverted repeats of a giant linear plasmid, SCP1.
MedLine Citation:
PMID:  1749818     Owner:  NLM     Status:  MEDLINE    
SCP1 is a giant linear plasmid of 350 kb coding for the methylenomycin biosynthetic genes in Streptomyces coelicolor. The unusually long terminal inverted repeats present on both ends of SCP1 were analyzed on the nucleotide sequence level. Analysis of six clones containing the terminal 0.35-kb XbaI fragment revealed a slight heterogeneity in the nucleotide sequences of the SCP1 ends. Moreover, it was indicated that this fragment contained seven palindromic inverted repeats and a GT-rich region in the 5'-end strand. The size of the terminal inverted repeats was determined to be 81 kb by the cloning and sequencing of their end-points. An insertion sequence, IS466 was shown to be present just at the end of the right terminal inverted repeat.
H Kinashi; M Shimaji-Murayama; T Hanafusa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plasmid     Volume:  26     ISSN:  0147-619X     ISO Abbreviation:  Plasmid     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1992-01-21     Completed Date:  1992-01-21     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  7802221     Medline TA:  Plasmid     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  123-30     Citation Subset:  IM    
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S70700;  S70701;  S70702;  S70703;  X55687;  X55688;  X55689;  X55690;  X56856;  X58180
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MeSH Terms
Anti-Bacterial Agents / biosynthesis*
Base Sequence
DNA, Bacterial / genetics
Molecular Sequence Data
Plasmids / genetics*
Repetitive Sequences, Nucleic Acid*
Restriction Mapping
Streptomyces / genetics*,  metabolism
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/DNA, Bacterial; 0/Peptides; 52775-76-5/methylenomycin A

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