Document Detail


Nucleotide regulation of goblet cells in human airway epithelial explants: normal exocytosis in cystic fibrosis.
MedLine Citation:
PMID:  8398169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulation of mucin secretion by airway goblet cells is poorly understood and the receptor-based regulatory mechanisms have not been described in human airways. In the present study, we report that extracellular triphosphate nucleotides regulate the rate of granule release from goblet cells in both normal and cystic fibrosis (CF) airway epithelial explants. Explants isolated from nasal and tracheobronchial tissues were mounted in perfusion chambers and the secretory activity was assessed by videomicroscopic determination of degranulation in single goblet cells and by ELISA determination of mucins secreted into the mucosal perfusate. Baseline degranulation was measured at 0.05 degranulation events (DE)/min. In normal goblet cells, mucosal ATP (10(-4) M, n = 17) induced a biphasic secretory response comprising 29.1 +/- 4.9 DE during the first 5 min, with an initial rate of 118.2 +/- 10.2 DE/min. Mucosal UTP (10(-4) M, n = 9) induced a similar response to ATP (initial rate: 89.2 +/- 23.9 DE/min, 17.9 +/- 5.1 DE in 5 min), but mucosal 2-MeSATP was not an effective agonist (initial rate: 1.5 +/- 1.4 DE/min, 2.3 +/- 0.5 DE in 5 min). Determination of mucins by ELISA confirmed that both ATP and UTP induced similar secretory responses but that 2-MeSATP was not effective. In CF explants, mucosal UTP (10(-4) M, n = 6) induced similar responses to those observed in normal tissues (initial rate: 82.5 +/- 27.5 DE/min, 18.8 +/- 4.1 DE in 5 min). We conclude that human nasal and tracheobronchial goblet cells are stimulated by mucosal nucleotides, probably via a 5'-nucleotide receptor, and that this response is unaffected by CF.
Authors:
M I Lethem; M L Dowell; M Van Scott; J R Yankaskas; T Egan; R C Boucher; C W Davis
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  9     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-10-26     Completed Date:  1993-10-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  315-22     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of North Carolina, Chapel Hill.
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MeSH Terms
Descriptor/Qualifier:
Bronchi / cytology*,  metabolism
Culture Techniques
Cystic Fibrosis / metabolism*
Enzyme-Linked Immunosorbent Assay
Epithelial Cells
Epithelium / metabolism
Exocytosis*
Humans
Nasal Mucosa / cytology*,  metabolism
Nucleotides / metabolism*
Trachea / cytology*,  metabolism
Grant Support
ID/Acronym/Agency:
HL34322/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Nucleotides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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