Document Detail


Nucleotide exchange factors for Hsp70s are required for [URE3] prion propagation in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  17392510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The [URE3] and [PSI(+)] prions are infectious amyloid forms of Ure2p and Sup35p. Several chaperones influence prion propagation: Hsp104p overproduction destabilizes [PSI(+)], whereas [URE3] is sensitive to excess of Ssa1p or Ydj1p. Here, we show that overproduction of the chaperone, Sse1p, can efficiently cure [URE3]. Sse1p and Fes1p are nucleotide exchange factors for Ssa1p. Interestingly, deletion of either SSE1 or FES1 completely blocked [URE3] propagation. In addition, deletion of SSE1 also interfered with [PSI(+)] propagation.
Authors:
Dmitry Kryndushkin; Reed B Wickner
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2007-03-28
Journal Detail:
Title:  Molecular biology of the cell     Volume:  18     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-25     Completed Date:  2007-10-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2149-54     Citation Subset:  IM    
Affiliation:
Laboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / genetics,  metabolism
Glutathione Peroxidase
Guanine Nucleotide Exchange Factors / genetics,  metabolism*
HSP110 Heat-Shock Proteins / genetics,  metabolism*
HSP40 Heat-Shock Proteins / genetics,  metabolism
HSP70 Heat-Shock Proteins / genetics,  metabolism*
Intracellular Signaling Peptides and Proteins / genetics,  metabolism
Point Mutation
Prions / genetics,  metabolism*
Saccharomyces cerevisiae / cytology,  genetics,  metabolism*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/FES1 protein, S cerevisiae; 0/Guanine Nucleotide Exchange Factors; 0/HSP110 Heat-Shock Proteins; 0/HSP40 Heat-Shock Proteins; 0/HSP70 Heat-Shock Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Prions; 0/SSE1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 139874-78-5/YDJ1 protein, S cerevisiae; EC 1.11.1.9/Glutathione Peroxidase; EC 1.11.1.9/URE2 protein, S cerevisiae; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.3/SSA1 protein, S cerevisiae
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  RNAi knockdown of Nopp140 induces Minute-like phenotypes in Drosophila.
Next Document:  Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol deplet...