Document Detail

Nucleoside analogue electrochemical behaviour and in situ evaluation of DNA-clofarabine interaction.
MedLine Citation:
PMID:  21856252     Owner:  NLM     Status:  MEDLINE    
Nucleoside analogues have had a substantial impact on the treatment of cancer, especially haematological malignancies. The electrochemical behaviour of the nucleoside analogue clofarabine (CLF) was investigated at a glassy carbon electrode using cyclic, differential pulse and square wave voltammetry in different pH supporting electrolytes. The oxidation process of CLF is irreversible and pH-dependent with transfer of two protons and two electrons, following a diffusion controlled mechanism. The oxidation mechanism of CLF involves deprotonation and leads to the formation of a hydroxylated species that undergoes reversible redox reactions. The interaction of DNA and the antileukemia drug CLF was investigated using a dsDNA-electrochemical biosensor in incubated solutions by differential pulse voltammetry. The CLF-DNA interaction leads to changes in the DNA morphological structure, confirmed using the purinic homo-polynucleotide single stranded sequences of guanosine and adenosine, poly[G] and poly[A]-electrochemical biosensors.
H Eda Satana; A Dora R Pontinha; Victor C Diculescu; Ana Maria Oliveira-Brett
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-27
Journal Detail:
Title:  Bioelectrochemistry (Amsterdam, Netherlands)     Volume:  87     ISSN:  1878-562X     ISO Abbreviation:  Bioelectrochemistry     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-08-20     Completed Date:  2013-01-07     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  100953583     Medline TA:  Bioelectrochemistry     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Coimbra, Portugal.
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MeSH Terms
Adenine Nucleotides / chemistry*
Antineoplastic Agents / chemistry*
Arabinonucleosides / chemistry*
DNA / chemistry*
Electrochemical Techniques
Hydrogen-Ion Concentration
Nucleic Acid Conformation
Poly A / chemistry*
Poly G / chemistry*
Reg. No./Substance:
0/Adenine Nucleotides; 0/Antineoplastic Agents; 0/Arabinonucleosides; 0/Electrolytes; 0/Protons; 24937-83-5/Poly A; 25191-14-4/Poly G; 7440-44-0/Carbon; 762RDY0Y2H/clofarabine; 9007-49-2/DNA

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