| Nucleoporin levels regulate cell cycle progression and phase-specific gene expression. | |
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MedLine Citation:
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PMID: 19000832 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Nup107-160 complex, the largest subunit of the nuclear pore, is multifunctional. It mediates mRNA export in interphase, and has roles in kinetochore function, spindle assembly, and postmitotic nuclear pore assembly. We report here that the levels of constituents of the Nup107-160 complex are coordinately cell cycle-regulated. At mitosis, however, a member of the complex, Nup96, is preferentially downregulated. This occurs via the ubiquitin-proteasome pathway. When the levels of Nup96 are kept high, a significant delay in G1/S progression occurs. Conversely, in cells of Nup96(+/-) mice, which express low levels of Nup96, cell cycle progression is accelerated. These lowered levels of Nup96 yield specific defects in nuclear export of certain mRNAs and protein expression, among which are key cell cycle regulators. Thus, Nup96 levels regulate differential gene expression in a phase-specific manner, setting the stage for proper cell cycle progression. |
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Authors:
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Papia Chakraborty; Yaming Wang; Jen-Hsuan Wei; Jan van Deursen; Hongtao Yu; Liviu Malureanu; Mary Dasso; Douglass J Forbes; David E Levy; Joachim Seemann; Beatriz M A Fontoura |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Developmental cell Volume: 15 ISSN: 1878-1551 ISO Abbreviation: Dev. Cell Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-12 Completed Date: 2009-02-11 Revised Date: 2011-03-30 |
Medline Journal Info:
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Nlm Unique ID: 101120028 Medline TA: Dev Cell Country: United States |
Other Details:
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Languages: eng Pagination: 657-67 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cell Line Down-Regulation* Gene Expression Hela Cells Humans Mice Mitosis Nuclear Pore / metabolism Nuclear Pore Complex Proteins / metabolism* RNA, Messenger / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM033279-25/GM/NIGMS NIH HHS; R01 GM067159-08/GM/NIGMS NIH HHS; R01AI28900/AI/NIAID NIH HHS; R01GM07159-01/GM/NIGMS NIH HHS; U54AI5715801/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nuclear Pore Complex Proteins; 0/RNA, Messenger; 0/nuclear pore complex protein 96 |
| Comments/Corrections | |
Comment In:
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Dev Cell. 2008 Nov;15(5):643-4
[PMID:
19000830
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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