Document Detail

Nucleolus organizers in Mus musculus subspecies and in the RAG mouse cell line.
MedLine Citation:
PMID:  69563     Owner:  NLM     Status:  MEDLINE    
Silver staining has been used to detect active nucleolus organizer regions (NOR's). By this criterion six mouse chromosomes, numbers 12, 15, 16, 17, 18 and 19, can have an NOR. The number and distribution of chromosomes with NOR's vary among inbred strains of Mus musculus musculus (C57BL/6J, BALB/cJ, C3H/HeJ and C3H/StCpr1BR) and in M. musculus molossinus. In a musculus X molassinus F1 hybrid, nucleolus organizers from each parent are silver stained.--Chromosomes which have NOR's in diploid cells also show them in tetraploid cells and in established cell lines. The BALB/cJ strain shows Ag-staining of NOR's on chromosomes 12, 15, 18 and occasionally 16. In the RAG cell line, which was derived from BALB/c, active NOR's are seen on 12, 15 and 18, even after these chromosomes have undergone structural rearrangements in the cell line. Some correlation exists between the amount of Ag-stain and the size of a secondary construction region, with a large amount of Ag-stain present on a chromosome which has a prominent secondary constriction. There is no correlation between the amount of Ag-stain and the presence or absence of C-band material.
V G Dev; R Tantravahi; D A Miller; O J Miller
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  86     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1977 Jun 
Date Detail:
Created Date:  1977-09-17     Completed Date:  1977-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  389-98     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Nucleolus / ultrastructure*
Chromosome Mapping
Chromosomes / ultrastructure*
Mice / anatomy & histology*
Mice, Inbred Strains
RNA, Ribosomal / biosynthesis
Staining and Labeling
Reg. No./Substance:
0/RNA, Ribosomal; 7440-22-4/Silver

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