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Nucleoli of diploid cell strains. Thei normal ultrastructure and the effects of toyocamycin and actinomycin D.
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MedLine Citation:
PMID:  5104724     Owner:  NLM     Status:  MEDLINE    
Nucleoli of cultured Chinese hamster or mouse cells in early passages had a loosely reticular substructure. Within the reticulum small, irregularly shaped, light fibrillar zones occurred which were contiguous with denser fibrillar zones. These denser zones appeared to be connected in some places to the particulate material which composed the mass of the nucleolus. Generally, electron-transparent spaces separated the particulate zones from the fibrillar areas. Treatment with toyocamycin, an agent which is reported to cause a blockage in the processing of ribosomal RNA, greatly inhibited the accumulation of newly synthesized RNA in the cytoplasm, as monitored by radioautography. Toyocamycin treatment caused the gradual disappearance of the granules from the particulate region of the nucleoli, and resulted ultimately in the nucleoli appearing homogeneously fibrillar. Actinomycin D treatment, which inhibited virtually all RNA synthesis, caused a segregation, and finally a disaggregation, of nucleolar components.
S G Phillips; D M Phillips
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  49     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1971 Jun 
Date Detail:
Created Date:  1971-08-23     Completed Date:  1971-08-23     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  785-802     Citation Subset:  IM    
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MeSH Terms
Antibiotics, Antineoplastic / pharmacology*
Cell Line
Cell Nucleolus* / analysis,  drug effects*
Culture Techniques*
Cytoplasm / analysis,  metabolism
Dactinomycin / pharmacology*
Microscopy, Electron
Pyrimidines / pharmacology*
Pyrroles / pharmacology
RNA / analysis,  antagonists & inhibitors,  biosynthesis*
RNA, Ribosomal / biosynthesis
Ribose / pharmacology
Uridine / metabolism
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Pyrimidines; 0/Pyrroles; 0/RNA, Ribosomal; 10028-17-8/Tritium; 50-69-1/Ribose; 50-76-0/Dactinomycin; 58-96-8/Uridine; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1971 by The Rockefeller University Press
Received Day: 31 Month: 8 Year: 1970
Revision Received Day: 20 Month: 10 Year: 1970
Print publication date: Day: 1 Month: 6 Year: 1971
Volume: 49 Issue: 3
First Page: 785 Last Page: 802
ID: 2108481
PubMed Id: 5104724

NUCLEOLI OF DIPLOID CELL STRAINS : Their Normal Ultrastructure and the Effects of Toyocamycin and Actinomycin D
Stephanie G. Phillips
David M. Phillips
From the Department of Biology, Washington University, St. Louis, Missouri 63130

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