| Nucleoli of diploid cell strains. Thei normal ultrastructure and the effects of toyocamycin and actinomycin D. | |
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MedLine Citation:
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PMID: 5104724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleoli of cultured Chinese hamster or mouse cells in early passages had a loosely reticular substructure. Within the reticulum small, irregularly shaped, light fibrillar zones occurred which were contiguous with denser fibrillar zones. These denser zones appeared to be connected in some places to the particulate material which composed the mass of the nucleolus. Generally, electron-transparent spaces separated the particulate zones from the fibrillar areas. Treatment with toyocamycin, an agent which is reported to cause a blockage in the processing of ribosomal RNA, greatly inhibited the accumulation of newly synthesized RNA in the cytoplasm, as monitored by radioautography. Toyocamycin treatment caused the gradual disappearance of the granules from the particulate region of the nucleoli, and resulted ultimately in the nucleoli appearing homogeneously fibrillar. Actinomycin D treatment, which inhibited virtually all RNA synthesis, caused a segregation, and finally a disaggregation, of nucleolar components. |
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Authors:
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S G Phillips; D M Phillips |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 49 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1971 Jun |
Date Detail:
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Created Date: 1971-08-23 Completed Date: 1971-08-23 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 785-802 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibiotics, Antineoplastic / pharmacology* Autoradiography Cell Line Cell Nucleolus* / analysis, drug effects* Cricetinae Culture Techniques* Cytoplasm / analysis, metabolism Dactinomycin / pharmacology* Diploidy* Fetus Histocytochemistry Mice Microscopy, Electron Pyrimidines / pharmacology* Pyrroles / pharmacology RNA / analysis, antagonists & inhibitors, biosynthesis* RNA, Ribosomal / biosynthesis Ribose / pharmacology Tritium Uridine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Pyrimidines; 0/Pyrroles; 0/RNA, Ribosomal; 10028-17-8/Tritium; 50-69-1/Ribose; 50-76-0/Dactinomycin; 58-96-8/Uridine; 63231-63-0/RNA |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1971 by The Rockefeller University Press Received Day: 31 Month: 8 Year: 1970 Revision Received Day: 20 Month: 10 Year: 1970 Print publication date: Day: 1 Month: 6 Year: 1971 Volume: 49 Issue: 3 First Page: 785 Last Page: 802 ID: 2108481 PubMed Id: 5104724 |
| NUCLEOLI OF DIPLOID CELL STRAINS : Their Normal Ultrastructure and the Effects of Toyocamycin and Actinomycin D | |
| Stephanie G. Phillips | |
| David M. Phillips | |
| From the Department of Biology, Washington University, St. Louis, Missouri 63130 |
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