|Nucleoli of diploid cell strains. Thei normal ultrastructure and the effects of toyocamycin and actinomycin D.|
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|PMID: 5104724 Owner: NLM Status: MEDLINE|
|Nucleoli of cultured Chinese hamster or mouse cells in early passages had a loosely reticular substructure. Within the reticulum small, irregularly shaped, light fibrillar zones occurred which were contiguous with denser fibrillar zones. These denser zones appeared to be connected in some places to the particulate material which composed the mass of the nucleolus. Generally, electron-transparent spaces separated the particulate zones from the fibrillar areas. Treatment with toyocamycin, an agent which is reported to cause a blockage in the processing of ribosomal RNA, greatly inhibited the accumulation of newly synthesized RNA in the cytoplasm, as monitored by radioautography. Toyocamycin treatment caused the gradual disappearance of the granules from the particulate region of the nucleoli, and resulted ultimately in the nucleoli appearing homogeneously fibrillar. Actinomycin D treatment, which inhibited virtually all RNA synthesis, caused a segregation, and finally a disaggregation, of nucleolar components.|
|S G Phillips; D M Phillips|
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|Type: Journal Article|
|Title: The Journal of cell biology Volume: 49 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1971 Jun|
|Created Date: 1971-08-23 Completed Date: 1971-08-23 Revised Date: 2010-06-22|
Medline Journal Info:
|Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES|
|Languages: eng Pagination: 785-802 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Antibiotics, Antineoplastic / pharmacology*
Cell Nucleolus* / analysis, drug effects*
Cytoplasm / analysis, metabolism
Dactinomycin / pharmacology*
Pyrimidines / pharmacology*
Pyrroles / pharmacology
RNA / analysis, antagonists & inhibitors, biosynthesis*
RNA, Ribosomal / biosynthesis
Ribose / pharmacology
Uridine / metabolism
|0/Antibiotics, Antineoplastic; 0/Pyrimidines; 0/Pyrroles; 0/RNA, Ribosomal; 10028-17-8/Tritium; 50-69-1/Ribose; 50-76-0/Dactinomycin; 58-96-8/Uridine; 63231-63-0/RNA|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Journal ID (nlm-ta): J Cell Biol
Publisher: The Rockefeller University Press
Copyright © 1971 by The Rockefeller University Press
Received Day: 31 Month: 8 Year: 1970
Revision Received Day: 20 Month: 10 Year: 1970
Print publication date: Day: 1 Month: 6 Year: 1971
Volume: 49 Issue: 3
First Page: 785 Last Page: 802
PubMed Id: 5104724
|NUCLEOLI OF DIPLOID CELL STRAINS : Their Normal Ultrastructure and the Effects of Toyocamycin and Actinomycin D|
|Stephanie G. Phillips|
|David M. Phillips|
|From the Department of Biology, Washington University, St. Louis, Missouri 63130
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