| Nucleolar localization signals of LIM kinase 2 function as a cell-penetrating peptide. | |
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MedLine Citation:
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PMID: 20937035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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LIM Kinase 2 (LIMK2) is a LIM domain-containing protein kinase which regulates actin polymerization thorough phosphorylation of the actin depolymerizing factor cofilin. It is also known to function as a shuttle between the cytoplasm and nucleus in endothelial cells. A basic amino acid-rich motif in LIMK2 was previously identified to be responsible for this shuttling function, as a nucleolar localization signal (NoLS). Here it is shown that this nucleolar localization signal sequence also has the characteristic function of a cell-penetrating peptide (CPP). We synthesized LIMK2 NoLS-conjugated peptides and a protein and analyzed their cell-penetrating abilities in various types of cells. The BC-box motif of the Von Hippel-Lindau (VHL) protein was used for the peptide. This motif previously has been reported to be involved in the neural differentiation of bone marrow stromal cells and skin-derived precursor cells. Green fluorescence protein (GFP) was used as a large biologically active biomolecule for the protein. The LIMK2 NoLS-conjugated peptides and protein translocated across the cell membranes of fibroblast cells, neural stem cells, and even iPS cells. These results suggest that LIMK2 NoLS acts as a cell-penetrating peptide and its cell-penetrating ability is not restricted by cell type. Moreover, from an in vivo assay using a mouse brain, it was confirmed that NoLS has potential for transporting biomolecules across the blood-brain barrier. |
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Authors:
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Nahoko Kobayashi; Mikio Niwa; Yang Hao; Tetsuhiko Yoshida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protein and peptide letters Volume: 17 ISSN: 1875-5305 ISO Abbreviation: Protein Pept. Lett. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-25 Completed Date: 2011-02-25 Revised Date: 2011-03-31 |
Medline Journal Info:
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Nlm Unique ID: 9441434 Medline TA: Protein Pept Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1480-8 Citation Subset: IM |
Affiliation:
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Institute for Advanced Sciences, Toagosei Co., Ltd., Tsukuba 300-2611, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Blood-Brain Barrier / metabolism Cell Nucleolus / metabolism* Cell-Penetrating Peptides / chemistry* Cells, Cultured Fibroblasts / metabolism Humans Infant, Newborn Lim Kinases / chemistry* Male Mice Mice, Inbred C57BL Molecular Sequence Data Neural Stem Cells / metabolism Nuclear Localization Signals / chemistry* Nuclear Proteins / chemistry*, metabolism Recombinant Fusion Proteins / chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cell-Penetrating Peptides; 0/Nuclear Localization Signals; 0/Nuclear Proteins; 0/Recombinant Fusion Proteins; EC 2.7.11.1/Lim Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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