Document Detail

Nucleocytoplasmic shuttling of the Rpb4p and Rpb7p subunits of Saccharomyces cerevisiae RNA polymerase II by two pathways.
MedLine Citation:
PMID:  17056745     Owner:  NLM     Status:  MEDLINE    
Rpb4p and Rpb7p are subunits of the RNA polymerase II of Saccharomyces cerevisiae that form a dissociable heterodimeric complex. Whereas the only reported function of Rpb7p is related to transcription, Rpb4p has been found to also act in mRNA export and in the major mRNA decay pathway that operates in the cytoplasm, thus raising the possibility that Rpb4p links between the nuclear and cytoplasmic processes. Here we show that both Rpb4p and Rpb7p shuttle between the nucleus and the cytoplasm. Shuttling kinetics of the two proteins are similar as long as their interaction is possible, suggesting that they shuttle as a heterodimer. Under normal conditions, shuttling of Rpb4p and Rpb7p depends on ongoing transcription. However, during severe stresses of heat shock, ethanol, and starvation, the two proteins shuttle via a transcription-independent pathway. Thus, Rpb4p and Rpb7p shuttle via two pathways, depending on environmental conditions.
Michael Selitrennik; Lea Duek; Rona Lotan; Mordechai Choder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-20
Journal Detail:
Title:  Eukaryotic cell     Volume:  5     ISSN:  1535-9778     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-12     Completed Date:  2007-01-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2092-103     Citation Subset:  IM    
Department of Molecular Microbiology, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel 31096.
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MeSH Terms
Active Transport, Cell Nucleus
Genes, Fungal
Heat-Shock Response
Models, Biological
Protein Subunits
RNA Polymerase II / chemistry*,  genetics,  metabolism*
RNA, Fungal / genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Recombinant Fusion Proteins / chemistry,  genetics,  metabolism
Saccharomyces cerevisiae / enzymology*,  genetics
Two-Hybrid System Techniques
Reg. No./Substance:
0/Protein Subunits; 0/RNA, Fungal; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; EC 2.7.7.-/RNA Polymerase II

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