Document Detail

Nucleobase catalysis in the hairpin ribozyme.
MedLine Citation:
PMID:  16601203     Owner:  NLM     Status:  MEDLINE    
RNA catalysis is important in the processing and translation of RNA molecules, yet the mechanisms of catalysis are still unclear in most cases. We have studied the role of nucleobase catalysis in the hairpin ribozyme, where the scissile phosphate is juxtaposed between guanine and adenine bases. We show that a modified ribozyme in which guanine 8 has been substituted by an imidazole base is active in both cleavage and ligation, with ligation rates 10-fold faster than cleavage. The rates of both reactions exhibit bell-shaped dependence on pH, with pK(a) values of 5.7 +/- 0.1 and 7.7 +/- 0.1 for cleavage and 6.1 +/- 0.3 and 6.9 +/- 0.3 for ligation. The data provide good evidence for general acid-base catalysis by the nucleobases.
Timothy J Wilson; Jonathan Ouellet; Zheng-Yun Zhao; Shinya Harusawa; Lisa Araki; Takushi Kurihara; David M J Lilley
Related Documents :
20873823 - Apolar versus polar solvents: a comparison of the strength of some organic acids agains...
14749073 - Chemical coupling between ammonia, acid gases, and fine particles.
21195763 - Juvenile hormone synthesis: "esterify then epoxidize" or "epoxidize then esterify"? ins...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-06
Journal Detail:
Title:  RNA (New York, N.Y.)     Volume:  12     ISSN:  1355-8382     ISO Abbreviation:  RNA     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-24     Completed Date:  2006-07-12     Revised Date:  2014-10-28    
Medline Journal Info:
Nlm Unique ID:  9509184     Medline TA:  RNA     Country:  United States    
Other Details:
Languages:  eng     Pagination:  980-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenosine / chemistry,  metabolism
Base Sequence
Binding Sites
Guanosine / chemistry,  metabolism
Hydrogen-Ion Concentration
Imidazoles / chemistry,  metabolism
Models, Molecular
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Catalytic / chemistry*,  metabolism
Grant Support
11722//Cancer Research UK
Reg. No./Substance:
0/Imidazoles; 0/RNA, Catalytic; 0/hairpin ribozyme; 12133JR80S/Guanosine; 7GBN705NH1/imidazole; K72T3FS567/Adenosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase.
Next Document:  Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA decay and translation.