| Nucleobase catalysis in the hairpin ribozyme. | |
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MedLine Citation:
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PMID: 16601203 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RNA catalysis is important in the processing and translation of RNA molecules, yet the mechanisms of catalysis are still unclear in most cases. We have studied the role of nucleobase catalysis in the hairpin ribozyme, where the scissile phosphate is juxtaposed between guanine and adenine bases. We show that a modified ribozyme in which guanine 8 has been substituted by an imidazole base is active in both cleavage and ligation, with ligation rates 10-fold faster than cleavage. The rates of both reactions exhibit bell-shaped dependence on pH, with pK(a) values of 5.7 +/- 0.1 and 7.7 +/- 0.1 for cleavage and 6.1 +/- 0.3 and 6.9 +/- 0.3 for ligation. The data provide good evidence for general acid-base catalysis by the nucleobases. |
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Authors:
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Timothy J Wilson; Jonathan Ouellet; Zheng-Yun Zhao; Shinya Harusawa; Lisa Araki; Takushi Kurihara; David M J Lilley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-06 |
Journal Detail:
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Title: RNA (New York, N.Y.) Volume: 12 ISSN: 1355-8382 ISO Abbreviation: RNA Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-24 Completed Date: 2006-07-12 Revised Date: 2010-09-15 |
Medline Journal Info:
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Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
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Languages: eng Pagination: 980-7 Citation Subset: IM |
Affiliation:
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Cancer Research UK Nucleic Acid Structure Research Group, The University of Dundee. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine
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chemistry,
metabolism Base Sequence Binding Sites Catalysis Guanosine / chemistry, metabolism Hydrogen-Ion Concentration Imidazoles / chemistry, metabolism Models, Molecular Molecular Sequence Data Nucleic Acid Conformation RNA, Catalytic / chemistry*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Imidazoles; 0/RNA, Catalytic; 0/hairpin ribozyme; 118-00-3/Guanosine; 288-32-4/imidazole; 58-61-7/Adenosine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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