Document Detail


Nucleic acids-based therapeutics in the battle against pathogenic viruses.
MedLine Citation:
PMID:  19048203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For almost three decades, researchers have studied the possibility to use nucleic acids as antiviral therapeutics. In theory, compounds such as antisense oligonucleotides, ribozymes, DNAzymes, and aptamers can be designed to trigger the sequence-specific inhibition of particular mRNA transcripts, including viral genomes. However, difficulties with their efficiency, off-target effects, toxicity, delivery, and stability halted the development of nucleic acid-based therapeutics that can be used in the clinic. So far, only a single antisense drug, Vitravene for the treatment of CMV-induced retinitis in AIDS patients, has made it to the clinic. Since the discovery of RNA interference (RNAi), there is a renewed interest in the development of nucleic acid-based therapeutics. Antiviral RNAi approaches are highly effective in vitro and in animal models and are currently being tested in clinical trials. Here we give an overview of antiviral nucleic acid-based therapeutics. We focus on antisense and RNAi-based compounds that have been shown to be effective in animal model systems.
Authors:
Joost Haasnoot; Ben Berkhout
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Handbook of experimental pharmacology     Volume:  -     ISSN:  0171-2004     ISO Abbreviation:  Handb Exp Pharmacol     Publication Date:  2009  
Date Detail:
Created Date:  2008-12-02     Completed Date:  2009-03-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902231     Medline TA:  Handb Exp Pharmacol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  243-63     Citation Subset:  IM    
Affiliation:
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam K3-110, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antiviral Agents / adverse effects,  pharmacology*,  therapeutic use*
Aptamers, Nucleotide / adverse effects,  therapeutic use
Humans
Nucleic Acids / adverse effects,  pharmacology*,  therapeutic use*
Oligonucleotides, Antisense / adverse effects,  pharmacology,  therapeutic use
RNA Interference / drug effects
RNA, Catalytic / adverse effects,  therapeutic use
RNA, Small Interfering / adverse effects,  therapeutic use
Virus Diseases / drug therapy*
Viruses / drug effects*
Chemical
Reg. No./Substance:
0/Antiviral Agents; 0/Aptamers, Nucleotide; 0/Nucleic Acids; 0/Oligonucleotides, Antisense; 0/RNA, Catalytic; 0/RNA, Small Interfering

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