| Nucleic acids analysis with nano-Ag-Tb(III) by a resonance light scattering technique. | |
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MedLine Citation:
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PMID: 16770061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The nano-Ag-terbium(III)-mucleic acids system was observed by a resonance light scattering (RLS) technique for the first time, and the quantitative analysis of nucleic acids at nanogram levels was established. Studies showed that the RLS intensity of the nano-Ag-terbium(III) system can be obviously enhanced by nucleic acid, which was characterized by the RLS spectrum and the UV-Vis spectrum. In this system, the nanoparticles were only of a definite size and in a limited particle concentration region. Further research indicated that under the optimum conditions, the enhanced intensity of RLS is in proportion to the concentration of nucleic acids in the ranges of 7.0 x 10(-9) g ml(-1) to 8.0 x 10(-6) g ml(-1) for calf thymus DNA (ctDNA), 2.0 x 10(-8) g ml(-1) to 1.0 x 10(-6) g ml(-1) for fish sperm DNA (fsDNA) and 1.0 x 10(-9) g ml(-1) to 1.0 x 10(-7) g ml(-1) for yeast RNA (yRNA). The detection limits were 1.4 ng ml(-1) for ctDNA, 1.2 ng ml(-1) for fsDNA and 0.85 ng ml(-1) for yRNA, respectively. Synthetic and real samples were determined satisfactorily. |
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Authors:
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Aiyun Li; Huichun Zhao; Linpei Jin; Dong Zheng |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry Volume: 22 ISSN: 0910-6340 ISO Abbreviation: Anal Sci Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-06-13 Completed Date: 2006-07-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8511078 Medline TA: Anal Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 775-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Beijing Normal University, P. R. China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calibration DNA / analysis* Nanostructures* RNA / analysis* Scattering, Radiation Sensitivity and Specificity Silver / chemistry* Spectrophotometry, Ultraviolet Terbium / chemistry* |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA; 7440-22-4/Silver; 7440-27-9/Terbium; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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