| Nucleic acid synthesis from blood urea 15N in the sheep. | |
| | |
MedLine Citation:
|
PMID: 6189137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In experiments on 4 sheep fed on a low protein diet [6.2 g N/day] and given a single i.v. dose of 15N-labelled urea [15 mg 15N/kg body mass], the authors found that, from 0.5 to 6 h, mean 15N incorporation rose progressively in the total rumen fluid nitrogen from 0.23 to 0.44 at. % 15N and in the rumen bacterial nitrogen from 0.11 to 0.51 at. % 15N. Up to 3 h, total nitrogen enrichment was greater (0.5 at. % 15N) than enrichment of bacterial nitrogen (0.28 at. % 15N), but from 3 to 6 h there was little difference between them. The mean 15N values in the nucleic acids isolated from rumen fluid bacteria in samples collected 3 and 6 hours after injecting labelled urea into the blood were 0.15 and 0.19 at. % 15N respectively, in nucleic acids isolated from the liver 0.042 and 0.04 at. % 15N, in the total rumen bacterial nitrogen 0.28 and 0.51 at. % 15N and in the total liver nitrogen 0.11 and 0.11 at. % 15N. It is concluded from the results that blood urea nitrogen is utilized for synthesis of the total nitrogenous substances of the sheep's rumen bacteria and liver far more intensively than for synthesis of the nucleic acids isolated from them. At the same time, it is utilized more intensively for nucleic acid synthesis in the rumen bacteria than in the liver. |
| | |
Authors:
|
P Javorský; E Rybosová; I Havassy; K Kosta |
Related Documents
:
|
6210687 - Extracellular acid proteases from neurospora crassa. 20118607 - Proline transport and stress tolerance of ammonia-insensitive mutants of the put4-encod... 3774737 - The effect of single essential amino acid deprivation on chick growth and nitrogen and ... 9739037 - Effects of xylitol on urea synthesis in patients with cirrhosis of the liver. 4033427 - Normal plasma free amino acid values in adults: the influence of some common physiologi... 15718827 - Effects of retinoic acid on retinal pigment epithelium from excised membranes from prol... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Physiologia Bohemoslovaca Volume: 32 ISSN: 0369-9463 ISO Abbreviation: Physiol Bohemoslov Publication Date: 1983 |
Date Detail:
|
Created Date: 1983-06-10 Completed Date: 1983-06-10 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0175317 Medline TA: Physiol Bohemoslov Country: CZECHOSLOVAKIA |
Other Details:
|
Languages: eng Pagination: 85-91 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bacteria / metabolism DNA / biosynthesis* Diet Liver / metabolism Male Nitrogen Isotopes RNA / biosynthesis* Sheep / metabolism* Urea / blood, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Nitrogen Isotopes; 57-13-6/Urea; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Changes in some digestive enzymes after a seven-day orbital flight.
Next Document: Maprotiline in the treatment of endogenous depression: comparison of therapeutic effect with seroton...