Document Detail


Nucleic acid synthesis from blood urea 15N in the sheep.
MedLine Citation:
PMID:  6189137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In experiments on 4 sheep fed on a low protein diet [6.2 g N/day] and given a single i.v. dose of 15N-labelled urea [15 mg 15N/kg body mass], the authors found that, from 0.5 to 6 h, mean 15N incorporation rose progressively in the total rumen fluid nitrogen from 0.23 to 0.44 at. % 15N and in the rumen bacterial nitrogen from 0.11 to 0.51 at. % 15N. Up to 3 h, total nitrogen enrichment was greater (0.5 at. % 15N) than enrichment of bacterial nitrogen (0.28 at. % 15N), but from 3 to 6 h there was little difference between them. The mean 15N values in the nucleic acids isolated from rumen fluid bacteria in samples collected 3 and 6 hours after injecting labelled urea into the blood were 0.15 and 0.19 at. % 15N respectively, in nucleic acids isolated from the liver 0.042 and 0.04 at. % 15N, in the total rumen bacterial nitrogen 0.28 and 0.51 at. % 15N and in the total liver nitrogen 0.11 and 0.11 at. % 15N. It is concluded from the results that blood urea nitrogen is utilized for synthesis of the total nitrogenous substances of the sheep's rumen bacteria and liver far more intensively than for synthesis of the nucleic acids isolated from them. At the same time, it is utilized more intensively for nucleic acid synthesis in the rumen bacteria than in the liver.
Authors:
P Javorský; E Rybosová; I Havassy; K Kosta
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Physiologia Bohemoslovaca     Volume:  32     ISSN:  0369-9463     ISO Abbreviation:  Physiol Bohemoslov     Publication Date:  1983  
Date Detail:
Created Date:  1983-06-10     Completed Date:  1983-06-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0175317     Medline TA:  Physiol Bohemoslov     Country:  CZECHOSLOVAKIA    
Other Details:
Languages:  eng     Pagination:  85-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / metabolism
DNA / biosynthesis*
Diet
Liver / metabolism
Male
Nitrogen Isotopes
RNA / biosynthesis*
Sheep / metabolism*
Urea / blood,  metabolism*
Chemical
Reg. No./Substance:
0/Nitrogen Isotopes; 57-13-6/Urea; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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