| Nucleic acid structure and sequence probing using fluorescent base analogue tC(O). | |
| | |
MedLine Citation:
|
PMID: 18963381 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The fluorescent cytosine analog tC(O) is on average the brightest probe of its kind and, moreover, it introduces minimal perturbations to the normal secondary structure of DNA. Here several ways of how tC(O), with an advantage, can be used as a local fluorescent probe in nucleic acid systems are presented. Most importantly, we show that tC(O) is an excellent probe for the detection of individual melting processes of complex nucleic acid structures containing a large number of separate secondary structure motifs. Since conventional UV-melting investigations merely monitor the global melting process of the whole nucleic acid structure, e.g. multi-hairpin systems in RNA/DNA, and thus is incapable of estimating individual melting transitions of such systems, tC(O) represents a new method of characterization. Furthermore, we find that tC(O) may be used to detect bulges and loops in nucleic acids as well as to distinguish a matched base-pair from several of the mismatched. |
| | |
Authors:
|
Karl Börjesson; Peter Sandin; L Marcus Wilhelmsson |
Publication Detail:
|
Type: Journal Article Date: 2008-10-11 |
Journal Detail:
|
Title: Biophysical chemistry Volume: 139 ISSN: 1873-4200 ISO Abbreviation: Biophys. Chem. Publication Date: 2009 Jan |
Date Detail:
|
Created Date: 2008-12-01 Completed Date: 2009-01-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0403171 Medline TA: Biophys Chem Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 24-8 Citation Subset: IM |
Affiliation:
|
Department of Chemical and Biological Engineering/Physical Chemistry, Chalmers University of Technology, SE-41296 Gothenburg, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Pair Mismatch Base Sequence Cytosine / analogs & derivatives DNA / chemistry* Fluorescent Dyes / chemistry* Freezing Inverted Repeat Sequences Nucleic Acid Conformation* Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes; 71-30-7/Cytosine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cellulose: a biopolymeric sorbent for heavy-metal traces in waters.
Next Document: Trace element analysis by particle and photon-induced X-ray emission spectroscopy.