Document Detail

Nucleic acid sandwich hybridization in detection of HPV 16 DNA: technique and its clinical application.
MedLine Citation:
PMID:  2832436     Owner:  NLM     Status:  MEDLINE    
Nucleic acid sandwich hybridization technique was used for detection of HPV 16 specific DNA in cervical scrapes. Alternating HPV 16-PstI fragments were cloned into plasmid pBR322 and phage M13mp10. pBR322-clones were used as 32P-labelled probe reagents and the M13mp10 clones as catching reagents in the assay. The detection limit of the test proved to be 1-5 X 10(5) HPV 16 molecules per test. A weak cross reaction was seen with HPV 31 DNA but not with the other types tested, e.g. HPV 6, 11, 18 and 33. Cervical scrapings obtained from 163 consecutive patients included in a prospective follow up study were analyzed for HPV 16 DNA with the sandwich hybridization method, dot-blot hybridization being used as a reference method. Sandwich hybridization assay detected 25 positive cases out of 163 specimens (15.3%). Of these 6 and 3 additional specimens were positive in dot-blot hybridization assay. HPV 16 DNA was related to higher PAP grades, and HPV 16 appeared more frequently in HPV CIN than HPV NCIN lesions. None of the infections caused by HPV 16 regressed, and 24% progressed during the follow up.
S Parkkinen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virological methods     Volume:  19     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-05-10     Completed Date:  1988-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-77     Citation Subset:  IM    
Department of Clinical Microbiology, University of Kuopio, Finland.
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MeSH Terms
Cervix Uteri / microbiology*
DNA, Viral / isolation & purification*
Nucleic Acid Hybridization*
Papillomaviridae / isolation & purification*
Tumor Virus Infections / diagnosis
Uterine Cervicitis / diagnosis
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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