Document Detail


Nucleic acid sandwich hybridization in detection of HPV 16 DNA: technique and its clinical application.
MedLine Citation:
PMID:  2832436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nucleic acid sandwich hybridization technique was used for detection of HPV 16 specific DNA in cervical scrapes. Alternating HPV 16-PstI fragments were cloned into plasmid pBR322 and phage M13mp10. pBR322-clones were used as 32P-labelled probe reagents and the M13mp10 clones as catching reagents in the assay. The detection limit of the test proved to be 1-5 X 10(5) HPV 16 molecules per test. A weak cross reaction was seen with HPV 31 DNA but not with the other types tested, e.g. HPV 6, 11, 18 and 33. Cervical scrapings obtained from 163 consecutive patients included in a prospective follow up study were analyzed for HPV 16 DNA with the sandwich hybridization method, dot-blot hybridization being used as a reference method. Sandwich hybridization assay detected 25 positive cases out of 163 specimens (15.3%). Of these 6 and 3 additional specimens were positive in dot-blot hybridization assay. HPV 16 DNA was related to higher PAP grades, and HPV 16 appeared more frequently in HPV CIN than HPV NCIN lesions. None of the infections caused by HPV 16 regressed, and 24% progressed during the follow up.
Authors:
S Parkkinen
Related Documents :
9504766 - Hpv 16 dna and mrna in cervical brush samples quantified by pcr and microwell hybridiza...
12658576 - Investigation of parrot papillomavirus in cloacal and oral papillomas of psittacine birds.
15459536 - Detection of human papillomavirus type 58 in polydactylous bowen's disease on the finge...
16176626 - Correlation between deoxyribonucleic acid loads of human papillomavirus and recurrence ...
15796306 - Electron beam irradiation as protection against the environmental release of recombinan...
11134086 - Human sperm telomere-binding complex involves histone h2b and secures telomere membrane...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virological methods     Volume:  19     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-05-10     Completed Date:  1988-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-77     Citation Subset:  IM    
Affiliation:
Department of Clinical Microbiology, University of Kuopio, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cervix Uteri / microbiology*
DNA, Viral / isolation & purification*
Female
Humans
Nucleic Acid Hybridization*
Papillomaviridae / isolation & purification*
Tumor Virus Infections / diagnosis
Uterine Cervicitis / diagnosis
Chemical
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A latex agglutination test for the detection of canine parvovirus and corresponding antibodies.
Next Document:  Effects of the antiglucocorticoid RU 486 on adrenal function in dogs.