| Nucleic acid and protein metabolism during the mitotic cycle in Vicia faba. | |
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MedLine Citation:
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PMID: 13786522 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to investigate some of the cytochemical processes involved in interphase growth and culminating in cell division, a combined autoradiographic and microphotometric study of nucleic acids and proteins was undertaken on statistically seriated cells of Vicia faba root meristems. Adenine-8-C(14) and uridine-H(3) were used as ribonucleic acid (RNA) precursors, thymidine-H(3) as a deoxyribonucleic acid (DNA) precursor, and phenylalanine-3-C(14) as a protein precursor. Stains used in microphotometry were Feulgen (DNA), azure B (RNA), pH 2.0 fast green (total protein), and pH 8.1 fast green (histone). The autoradiographic data (representing rate of incorporation per organelle) and the microphotometric data (representing changes in amounts of the various components) indicate that the mitotic cycle may be divided into several metabolic phases, three predominantly anabolic (net increase), and a fourth phase predominantly catabolic (net decrease). The anabolic periods are: 1. Telophase to post-telophase during which there are high rates of accumulation of cytoplasmic and nucleolar RNA and nucleolar and chromosomal total protein. 2. Post-telophase to preprophase characterized by histone synthesis and a diphasic synthesis of DNA with the peak of synthesis at mid-interphase and a minor peak just preceding prophase. The minor peak is coincident with a relatively localized DNA synthesis in several chromosomal regions. This period is also characterized by minimal accumulations of cytoplasmic RNA and chromosomal and nucleolar total protein and RNA. 3. Preprophase to prophase in which there are again high rates of accumulation of cytoplasmic RNA, and nucleolar and chromosomal total protein and RNA. The catabolic phase is: 4. The mitotic division during which there are marked losses of cytoplasmic RNA and chromosomal and nucleolar total protein and RNA. |
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Authors:
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J WOODARD; E RASCH; H SWIFT |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biophysical and biochemical cytology Volume: 9 ISSN: 0095-9901 ISO Abbreviation: J Biophys Biochem Cytol Publication Date: 1961 Feb |
Date Detail:
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Created Date: 1961-12-01 Completed Date: 1998-11-01 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 17840020R Medline TA: J Biophys Biochem Cytol Country: Not Available |
Other Details:
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Languages: eng Pagination: 445-62 Citation Subset: OM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division* Plants / metabolism* Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Biophys Biochem Cytol ISSN: 0095-9901 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © Copyright, 1961, by The Rockefeller Institute Press Received Day: 24 Month: 7 Year: 1960 Print publication date: Day: 1 Month: 2 Year: 1961 Volume: 9 Issue: 2 First Page: 445 Last Page: 462 ID: 2224997 PubMed Id: 13786522 |
| NUCLEIC ACID AND PROTEIN METABOLISM DURING THE MITOTIC CYCLE IN VICIA FABA | |
| John Woodard | |
| Ellen Rasch | |
| Hewson Swift | |
| From the Whitman Laboratory, Department of Zoology, University of Chicago |
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