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Nucleic acid and protein metabolism during the mitotic cycle in Vicia faba.
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MedLine Citation:
PMID:  13786522     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to investigate some of the cytochemical processes involved in interphase growth and culminating in cell division, a combined autoradiographic and microphotometric study of nucleic acids and proteins was undertaken on statistically seriated cells of Vicia faba root meristems. Adenine-8-C(14) and uridine-H(3) were used as ribonucleic acid (RNA) precursors, thymidine-H(3) as a deoxyribonucleic acid (DNA) precursor, and phenylalanine-3-C(14) as a protein precursor. Stains used in microphotometry were Feulgen (DNA), azure B (RNA), pH 2.0 fast green (total protein), and pH 8.1 fast green (histone). The autoradiographic data (representing rate of incorporation per organelle) and the microphotometric data (representing changes in amounts of the various components) indicate that the mitotic cycle may be divided into several metabolic phases, three predominantly anabolic (net increase), and a fourth phase predominantly catabolic (net decrease). The anabolic periods are: 1. Telophase to post-telophase during which there are high rates of accumulation of cytoplasmic and nucleolar RNA and nucleolar and chromosomal total protein. 2. Post-telophase to preprophase characterized by histone synthesis and a diphasic synthesis of DNA with the peak of synthesis at mid-interphase and a minor peak just preceding prophase. The minor peak is coincident with a relatively localized DNA synthesis in several chromosomal regions. This period is also characterized by minimal accumulations of cytoplasmic RNA and chromosomal and nucleolar total protein and RNA. 3. Preprophase to prophase in which there are again high rates of accumulation of cytoplasmic RNA, and nucleolar and chromosomal total protein and RNA. The catabolic phase is: 4. The mitotic division during which there are marked losses of cytoplasmic RNA and chromosomal and nucleolar total protein and RNA.
Authors:
J WOODARD; E RASCH; H SWIFT
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biophysical and biochemical cytology     Volume:  9     ISSN:  0095-9901     ISO Abbreviation:  J Biophys Biochem Cytol     Publication Date:  1961 Feb 
Date Detail:
Created Date:  1961-12-01     Completed Date:  1998-11-01     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  17840020R     Medline TA:  J Biophys Biochem Cytol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  445-62     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Cell Division*
Plants / metabolism*
Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Proteins
Comments/Corrections

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Full Text
Journal Information
Journal ID (nlm-ta): J Biophys Biochem Cytol
ISSN: 0095-9901
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1961, by The Rockefeller Institute Press
Received Day: 24 Month: 7 Year: 1960
Print publication date: Day: 1 Month: 2 Year: 1961
Volume: 9 Issue: 2
First Page: 445 Last Page: 462
ID: 2224997
PubMed Id: 13786522

NUCLEIC ACID AND PROTEIN METABOLISM DURING THE MITOTIC CYCLE IN VICIA FABA
John Woodard
Ellen Rasch
Hewson Swift
From the Whitman Laboratory, Department of Zoology, University of Chicago


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