| Nucleic acid pool preparation and characterization. | |
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MedLine Citation:
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PMID: 19377997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Random sequence nucleic acid pools can be used in a variety of applications, including the selection of functional nucleic acids such as protein binding sites, aptamers, and ribozymes. while the design, synthesis, and purification of pools is relatively straightforward, keeping track of the size and complexity of a nucleic acid pool can sometimes task even an experienced researcher. The following protocol takes the reader through the steps necessary for the preparation of a pool of known complexity. |
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Authors:
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Shawn K Piasecki; Bradley Hall; Andrew D Ellington |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 535 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-05-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 3-18 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aptamers, Nucleotide
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chemical synthesis,
chemistry,
genetics,
isolation & purification DNA Primers / chemical synthesis, genetics, isolation & purification Nucleic Acids / chemical synthesis*, chemistry, genetics, isolation & purification Polymerase Chain Reaction SELEX Aptamer Technique / methods* |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 0/DNA Primers; 0/Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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