Document Detail


Nucleic acid complexing glycosyl nucleoside-based amphiphile.
MedLine Citation:
PMID:  16029028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A neutral amphiphile derived from uridine featuring two oleyl chains and one glucose for DNA binding was prepared using a convenient four-step synthetic route. The nucleic acid binding capabilities of this amphiphile were investigated by UV-vis, quasi-elastic light scattering (QELS), transmission electronic microscopy (TEM), gel electrophoresis, 31P NMR, IR, and circular dichroism (CD). Amphiphile-nucleic acid complex formation is a consequence of the amphiphilic character of the molecule, phosphate-sugar, and nucleobase-nucleobase interactions. This work presents for the first time a glyco-nucleo-amphiphile capable of binding efficiently the nucleic acid double helix structure.
Authors:
Jerome Arigon; Carla A H Prata; Mark W Grinstaff; Philippe Barthélémy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  16     ISSN:  1043-1802     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:    2005 Jul-Aug
Date Detail:
Created Date:  2005-07-20     Completed Date:  2005-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  864-72     Citation Subset:  IM    
Affiliation:
Faculté des sciences d'Avignon, 33 rue Louis Pasteur, F-84000 Avignon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Electrophoresis, Agar Gel
Glucose / chemistry*
Microscopy, Electron, Transmission
Nucleic Acid Denaturation
Nucleic Acids / chemistry*
Nucleosides / chemistry*
Spectrum Analysis / methods
Chemical
Reg. No./Substance:
0/Nucleic Acids; 0/Nucleosides; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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