| Nucleic acid binding property of the gene products of rice stripe virus. | |
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MedLine Citation:
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PMID: 16025246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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GST fusion proteins of the six gene products from RNAs 2,3 and 4 of the tenuivirus, Rice stripe virus (RSV), were used to study the nucleic acid binding activities in vitro. Three of the proteins, p3, pc3 and pc4, bound both single- and double-stranded cDNA of RSV RNA4 and also RNA3 transcribed from its cDNA clone, while p2, pc2-N (the N-terminal part of pc2) nor p4 bound the cDNA or RNA transcript. The binding activity of p3 is located in the carboxyl-terminus amino acid 154-194, which contains basic amino acid rich beta-sheets. The acidic amino acid-rich amino-terminus (amino acids 1-100) of p3 did not have nucleic acid binding activity. The related analogous gene product of the tenuivirus, Rice hoja blanca virus, is a suppressor of gene silencing and the possibility of the nucleic acid binding ability of RSV p3 being associated with this property is discussed. The C-terminal part of the RSV nucleocapsid protein, which also contains a basic region, binds nucleic acids, which is consistent with its function. The central and C-terminal regions of pc4 bind nucleic acid. It has been suggested that this protein is a cell-to-cell movement protein and nucleic acid binding would be in accord with this function. |
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Authors:
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Delin Liang; Xiangqiang Ma; Zhicai Qu; Roger Hull |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virus genes Volume: 31 ISSN: 0920-8569 ISO Abbreviation: Virus Genes Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-07-18 Completed Date: 2006-09-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8803967 Medline TA: Virus Genes Country: United States |
Other Details:
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Languages: eng Pagination: 203-9 Citation Subset: IM |
Affiliation:
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John Innes Centre, Norwich Research Park, NR4 7UH Colney, Norwich, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Blotting, Southwestern Cloning, Molecular DNA, Complementary / metabolism DNA-Binding Proteins / chemistry, genetics, metabolism* Molecular Sequence Data Protein Structure, Tertiary RNA Interference RNA, Viral / metabolism RNA-Binding Proteins / chemistry, genetics, metabolism* Recombinant Fusion Proteins / metabolism Sequence Alignment Tenuivirus / chemistry, genetics, metabolism* Viral Proteins / chemistry, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/DNA-Binding Proteins; 0/RNA, Viral; 0/RNA-Binding Proteins; 0/Recombinant Fusion Proteins; 0/Viral Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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