Document Detail


Nucleic acid binding property of the gene products of rice stripe virus.
MedLine Citation:
PMID:  16025246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GST fusion proteins of the six gene products from RNAs 2,3 and 4 of the tenuivirus, Rice stripe virus (RSV), were used to study the nucleic acid binding activities in vitro. Three of the proteins, p3, pc3 and pc4, bound both single- and double-stranded cDNA of RSV RNA4 and also RNA3 transcribed from its cDNA clone, while p2, pc2-N (the N-terminal part of pc2) nor p4 bound the cDNA or RNA transcript. The binding activity of p3 is located in the carboxyl-terminus amino acid 154-194, which contains basic amino acid rich beta-sheets. The acidic amino acid-rich amino-terminus (amino acids 1-100) of p3 did not have nucleic acid binding activity. The related analogous gene product of the tenuivirus, Rice hoja blanca virus, is a suppressor of gene silencing and the possibility of the nucleic acid binding ability of RSV p3 being associated with this property is discussed. The C-terminal part of the RSV nucleocapsid protein, which also contains a basic region, binds nucleic acids, which is consistent with its function. The central and C-terminal regions of pc4 bind nucleic acid. It has been suggested that this protein is a cell-to-cell movement protein and nucleic acid binding would be in accord with this function.
Authors:
Delin Liang; Xiangqiang Ma; Zhicai Qu; Roger Hull
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus genes     Volume:  31     ISSN:  0920-8569     ISO Abbreviation:  Virus Genes     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-07-18     Completed Date:  2006-09-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803967     Medline TA:  Virus Genes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  203-9     Citation Subset:  IM    
Affiliation:
John Innes Centre, Norwich Research Park, NR4 7UH Colney, Norwich, UK.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Blotting, Southwestern
Cloning, Molecular
DNA, Complementary / metabolism
DNA-Binding Proteins / chemistry,  genetics,  metabolism*
Molecular Sequence Data
Protein Structure, Tertiary
RNA Interference
RNA, Viral / metabolism
RNA-Binding Proteins / chemistry,  genetics,  metabolism*
Recombinant Fusion Proteins / metabolism
Sequence Alignment
Tenuivirus / chemistry,  genetics,  metabolism*
Viral Proteins / chemistry,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/DNA-Binding Proteins; 0/RNA, Viral; 0/RNA-Binding Proteins; 0/Recombinant Fusion Proteins; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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