| Nucleic acid base analog FRET-pair facilitating detailed structural measurements in nucleic acid containing systems. | |
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MedLine Citation:
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PMID: 19317504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present the first nucleobase analog fluorescence resonance energy transfer (FRET)-pair. The pair consists of tC(O), 1,3-diaza-2-oxophenoxazine, as an energy donor and the newly developed tC(nitro), 7-nitro-1,3-diaza-2-oxophenothiazine, as an energy acceptor. The FRET-pair successfully monitors distances covering up to more than one turn of the DNA duplex. Importantly, we show that the rigid stacking of the two base analogs, and consequently excellent control of their exact positions and orientations, results in a high control of the orientation factor and hence very distinct FRET changes as the number of bases separating tC(O) and tC(nitro) is varied. A set of DNA strands containing the FRET-pair at wisely chosen locations will, thus, make it possible to accurately distinguish distance- from orientation-changes using FRET. In combination with the good nucleobase analog properties, this points toward detailed studies of the inherent dynamics of nucleic acid structures. Moreover, the placement of FRET-pair chromophores inside the base stack will be a great advantage in studies where other (biomacro)molecules interact with the nucleic acid. Lastly, our study gives possibly the first truly solid experimental support to the dependence of energy transfer efficiency on orientation of involved transition dipoles as predicted by the Forster theory. |
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Authors:
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Karl Börjesson; Søren Preus; Afaf H El-Sagheer; Tom Brown; Bo Albinsson; L Marcus Wilhelmsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 131 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-25 Completed Date: 2009-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 4288-93 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biological Engineering/Physical Chemistry, Chalmers University of Technology, S-41296 Gothenburg, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytosine
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analogs & derivatives,
chemistry DNA / chemistry Fluorescence Resonance Energy Transfer / methods* Models, Chemical Models, Theoretical Nitro Compounds / chemistry* Nucleic Acid Conformation Nucleic Acids / chemistry Oligonucleotides / chemistry Oxazines / chemistry* Phenothiazines / chemistry* Photochemistry / methods Spectrophotometry, Ultraviolet / methods Temperature |
| Chemical | |
Reg. No./Substance:
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0/1,3-diaza-2-oxophenoxazine; 0/7-nitro-1,3-diaza-2-oxophenothiazine; 0/Nitro Compounds; 0/Nucleic Acids; 0/Oligonucleotides; 0/Oxazines; 0/Phenothiazines; 71-30-7/Cytosine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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