| Nucleic acid assay system for tier II laboratories and moderately complex clinics to detect HIV in low-resource settings. | |
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MedLine Citation:
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PMID: 20225946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is a clear need for an instrument-free molecular diagnostic system for detecting human immunodeficiency virus type 1 (HIV-1) RNA or DNA that can be used in developing countries. Such a test could be used for early diagnosis of HIV-1 infection during infancy and could serve as a surrogate end point for vaccine trials. We developed the IsoAmp HIV-1 assay (BioHelix Corporation), which targets the HIV-1 gag gene with use of isothermal reverse-transcription helicase-dependent amplification chemistry. The IsoAmp HIV assay uses a disposable amplicon containment device with an embedded vertical-flow DNA detection strip to detect the presence of HIV-1 amplicons. The vertical-flow DNA detection strip has a control line to validate the performance of the device and a test line to detect the analyte. The analyte is detected by a sandwich immunoassay for reporter moieties on a capture probe and a detection probe. The control line consists of the detection probe reporter moiety conjugated to the vertical-flow DNA detection strip. The preliminary limit of detection of the IsoAmp HIV assay was evaluated by testing serial dilutions of HIV-1 armored RNA (Assuragen). We found that 21 (75%) of 28 assays yielded positive results when 50 copies of HIV-1 armored RNA were input into the IsoAmp HIV reaction. |
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Authors:
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Wen Tang; Wing Huen A Chow; Ying Li; Huimin Kong; Yi-Wei Tang; Bertrand Lemieux |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 201 Suppl 1 ISSN: 1537-6613 ISO Abbreviation: J. Infect. Dis. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-15 Completed Date: 2010-04-19 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: S46-51 Citation Subset: AIM; IM |
Affiliation:
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BioHelix Corporation, Beverly, Massachusetts, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Viral
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genetics,
isolation & purification HIV / genetics, isolation & purification* HIV Infections / diagnosis*, virology* Humans Immunoassay / methods Molecular Diagnostic Techniques / methods* Nucleic Acid Amplification Techniques / methods* RNA, Viral / genetics, isolation & purification Sensitivity and Specificity |
| Grant Support | |
ID/Acronym/Agency:
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1R43AI066487-01/AI/NIAID NIH HHS; 1R43AI77418-01/AI/NIAID NIH HHS; R43 AI077418-01/AI/NIAID NIH HHS; R44 AI077418-02/AI/NIAID NIH HHS; R44 AI077418-03/AI/NIAID NIH HHS; R44 AI077418-04/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/RNA, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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