| Nucleic acid aptamers targeting cell surface proteins. | |
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MedLine Citation:
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PMID: 21300154 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aptamers are chemical antibodies that bind to their targets with high affinity and specificity. These short stretches of nucleic acids are identified using a repetitive in vitro selection and partitioning technology called SELEX (Systematic Evolution of Ligands by EXponential enrichment). Since the emergence of this technology, many modifications and variations have been introduced to enable the selection of specific ligands, even for implausible targets. For membrane protein, the selection scheme can be chosen depending upon the availability of the system, the protein characteristics and the application required. Aptamers have been generated for a significant number of disease-associated membrane proteins and have been shown to have considerable diagnostic and therapeutic importance. In this article, we review the SELEX process used for identification of aptamers that target cell surface proteins and recapitulate their use as therapeutic and diagnostic reagents. |
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Authors:
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Pooja Dua; Soyoun Kim; Dong-Ki Lee |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-4 |
Journal Detail:
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Title: Methods (San Diego, Calif.) Volume: - ISSN: 1095-9130 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9426302 Medline TA: Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
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Department of Biomedical Engineering, Dongguk University, Seoul Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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