| Nucleic acid amplification mediated microbial identification. | |
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MedLine Citation:
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PMID: 7536958 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acid amplification techniques have and are revolutionising the way research, academia, medicine, environmental and quality control issues are dealt with. In particular, the polymerase chain reaction, (PCR), originally mentioned in 1985 has greatly modified the ways in which scientists and clinicians go about nucleic acid sequence analysis and manipulation and has great implications for molecular biology in general and diagnostics in particular. This report attempts to review the use of PCR and isothermal nucleic acid amplification techniques in the rapid identification of microorganisms. Key methodologies have been noted, their strengths and weaknesses commented upon and comparisons have been drawn between isothermal and thermally cycled nucleic amplification techniques in rapid microbial identification. |
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Authors:
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I J Bruce |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Science progress Volume: 77 ( Pt 3-4) ISSN: 0036-8504 ISO Abbreviation: Sci Prog Publication Date: 1993-1994 |
Date Detail:
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Created Date: 1995-05-25 Completed Date: 1995-05-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0411361 Medline TA: Sci Prog Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 183-206 Citation Subset: IM |
Affiliation:
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University of Greenwich, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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genetics* Bacterial Infections / diagnosis, microbiology Base Sequence DNA, Bacterial / genetics* DNA, Ribosomal / genetics Escherichia coli / genetics Humans Molecular Sequence Data Polymerase Chain Reaction / methods* RNA, Bacterial / genetics* RNA, Ribosomal, 16S / genetics Site-Specific DNA-Methyltransferase (Adenine-Specific) / genetics Software |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; EC 2.1.1.-/DNA modification methylase EcoRI; EC 2.1.1.72/Site-Specific DNA-Methyltransferase (Adenine-Specific) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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