| Nucleic acid amplification-based techniques for pathogen detection and identification. | |
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MedLine Citation:
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PMID: 16169776 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed. |
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Authors:
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Paul T Monis; Steven Giglio |
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Publication Detail:
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Type: Journal Article; Review Date: 2005-09-19 |
Journal Detail:
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Title: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases Volume: 6 ISSN: 1567-1348 ISO Abbreviation: Infect. Genet. Evol. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2005-12-26 Completed Date: 2006-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101084138 Medline TA: Infect Genet Evol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 2-12 Citation Subset: IM |
Affiliation:
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Australian Water Quality Centre, South Australian Water Corporation, Microbiology Unit, Salisbury, SA 5108, Australia. paul.monis@sawater.com.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Bacterial
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analysis DNA, Viral / analysis*, metabolism Molecular Biology / methods Molecular Diagnostic Techniques* / instrumentation Nucleic Acid Amplification Techniques* Nucleic Acid Conformation Nucleic Acid Hybridization Virulence Factors / genetics |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Viral; 0/Virulence Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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