| Nucleic acids electrotransfer in vivo: mechanisms and practical aspects. | |
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MedLine Citation:
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PMID: 20557285 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acids transfer has been steadily improving over the years and is slowly starting to fulfill its long awaited promises. In the beginning, viral approaches raised strong safety concerns that are now answered by various non-viral techniques. Among the physical approaches developed, nucleic acids electrotransfer is probably the one with the highest momentum. Here we review the present knowledge on the mechanistic and practical aspects of in vivo nucleic acids electrotransfer. For each step of this procedure we present different strategies that are used, with their advantages and drawbacks. As we report here, practical solutions have been found to overcome each limiting step in the procedure and to improve its outcome. Some crucial issues are beyond the application of the electric pulses itself, like the administration (i.e., in almost all of the cases, the injection) of the nucleic acids to the tissue or the body. High efficiency and safety are at reach if all the present knowledge and strategies are put to use. Electrotransfer is now a mature technique as proven by the fact that clinical trials using nucleic acids electrotransfer have already started within the past few years. |
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Authors:
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Franck M Andre; Lluis M Mir |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Current gene therapy Volume: 10 ISSN: 1875-5631 ISO Abbreviation: Curr Gene Ther Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-19 Completed Date: 2010-12-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101125446 Medline TA: Curr Gene Ther Country: Netherlands |
Other Details:
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Languages: eng Pagination: 267-80 Citation Subset: IM |
Affiliation:
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CNRS, UMR 8203-Institut Gustave Roussy PR2, 114 rue Edouard Vaillant, Villejuif, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breast Neoplasms / therapy Cell Membrane / physiology Cell Membrane Permeability / physiology DNA / administration & dosage, metabolism* Electrodes Electroporation* Extracellular Matrix / metabolism Female Gene Expression / physiology Gene Therapy* Gene Transfer Techniques* Humans Melanoma / therapy Mice RNA / administration & dosage, metabolism* Rabbits Rats Skin Neoplasms / therapy Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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