| Nucleic Acid-based Methods to Assess the Composition and Function of the Bowel Microbiota. | |
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MedLine Citation:
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PMID: 23101691 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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This article describes nucleic acid-based methods to assess the composition and function of the bowel microbiota. The methods range from the relatively simple (polymerase chain reaction) to the technically sophisticated (metatranscriptomics). Not all are accessible to the majority of laboratories, but a core of validated (used in more than 1 study) tools is readily available to most researchers. Reliance on a single methodology per human study is not recommended. Generally, a study could commence with a screening of samples to determine whether it will be worthwhile expending further time and money on an in-depth analysis. |
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Authors:
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Blair Lawley; Gerald W Tannock |
Publication Detail:
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Type: Journal Article Date: 2012-09-28 |
Journal Detail:
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Title: Gastroenterology clinics of North America Volume: 41 ISSN: 1558-1942 ISO Abbreviation: Gastroenterol. Clin. North Am. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-10-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8706257 Medline TA: Gastroenterol Clin North Am Country: United States |
Other Details:
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Languages: eng Pagination: 855-68 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Microbiology and Immunology, University of Otago, Post Office Box 56, 720 Cumberland Street, Dunedin, New Zealand. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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