Document Detail

Nucleic Acid Driven DNA Machineries Synthesizing Mg(2+) -Dependent DNAzymes: An Interplay between DNA Sensing and Logic-Gate Operations.
MedLine Citation:
PMID:  23015539     Owner:  NLM     Status:  Publisher    
Polymerase/nicking enzymes and nucleic-acid scaffolds are implemented as DNA machines for the development of amplified DNA-detection schemes, and for the design of logic gates. The analyte nucleic acid target acts, also, as input for the logic gates. In the presence of two DNA targets, acting as inputs, and appropriate DNA scaffolds, the polymerase-induced replication of the scaffolds, followed by the nicking of the replication products, are activated, leading to the autonomous synthesis of the Mg(2+) -dependent DNAzyme or the Mg(2+) -dependent DNAzyme subunits. These biocatalysts cleave a fluorophore/quencher-functionalized nucleic-acid substrate, thus providing fluorescence signals for the sensing events or outputs for the logic gates. The systems are used to develop OR, AND, and Controlled-AND gates, and the DNA-analyte targets represent two nucleic acid sequences of the smallpox viral genome.
Ron Orbach; Lena Mostinski; Fuan Wang; Itamar Willner
Related Documents :
15047689 - Role of atp hydrolysis in the antirecombinase function of saccharomyces cerevisiae srs2...
23352269 - Synthesis and enzymatic incorporation of α-l-threofuranosyl adenine triphosphate (tatp).
24984849 - Resources for small regulatory rnas.
24101469 - Dna supercoiling enhances cooperativity and efficiency of an epigenetic switch.
23125969 - Positively charged polymer brush-functionalized filter paper for dna sequence determina...
3899189 - Role of escherichia coli reca protein in sos induction and post-replication repair.
24306989 - Extrachromosomal dna of pea-root (pisum sativum) has repeated sequences and ribosomal g...
18425479 - Arrayed primer extension reaction for genotyping on oligonucleotide microarray.
9568229 - Hypertrophic cardiomyopathy with mitochondrial dna depletion and respiratory enzyme def...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Chemistry (Weinheim an der Bergstrasse, Germany)     Volume:  -     ISSN:  1521-3765     ISO Abbreviation:  Chemistry     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9513783     Medline TA:  Chemistry     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Institute of Chemistry, The Minerva Center for Complex Biohybrid Systems, The Hebrew University of Jerusalem, Jerusalem 91904 (Israel), Fax: (+972) 2-652-7715.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Low dose of carbon monoxide intraperitoneal injection provides potent protection against GalN/LPS-in...
Next Document:  3D Bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels.