| Nuclei and subnuclei gene expression profiling in mammalian brain. | |
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MedLine Citation:
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PMID: 12088837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Information on the neuroanatomical expression of a given gene is critical to understanding its function in the central nervous system. The integration of laser capture microdissection (LCM), T7-based RNA amplification and cDNA microarrays allows for this information to be simultaneously generated for thousands of genes. To validate this integrative approach, we catalogued the gene expression profiles of seven rat brain nuclei or subnuclei. A hundred cells from the following seven brain nuclei were analyzed: locus coeruleus (LC), dorsal raphe nucleus (DR), parvocellular division (PA) and magnocellular division (MG) of the hypothalamic paraventricular nucleus (PVN) and CA1, CA3 and dentate gyrus (DG) divisions of the hippocampal formation. Of the 2145 genes investigated, 1402 genes (65%) gave a hybridization signal statistically different from the background level that was defined by non-specific hybridizations to 15 different plant genes. Validation of our microarray data on four arbitrarily selected genes was confirmed by Real-Time PCR. Previous research showing expression patterns of 'signature' genes (n=17) for specific brain nuclei are consistent with our findings. For example, as previously shown, enriched mRNA expression encoding the serotonin transporter or tyrosine hydroxylase was found in DR and LC cells, respectively. Interestingly, expression of the serotonin 5-HT(2B) receptor mRNA was also found in DR cells. We confirmed this new finding by in-situ hybridization. The hierarchical clustering analysis of gene expression shows that the two divisions of the PVN (PA and MG) are closely related to each other, as well as the three regions of the hippocampal formation (CA1, CA3 and DG), which also showed similar gene expression profiles. This study demonstrates the importance, feasibility and utility of cellular brain nuclei profiling. |
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Authors:
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Pascal Bonaventure; Hongqing Guo; Bin Tian; Xuejun Liu; Anton Bittner; Barbara Roland; Ranelle Salunga; Xiao-Jun Ma; Fredrik Kamme; Bernhard Meurers; Margot Bakker; Mirek Jurzak; Josée E Leysen; Mark G Erlander |
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Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: Brain research Volume: 943 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-06-28 Completed Date: 2002-10-02 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 38-47 Citation Subset: IM |
Affiliation:
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Johnson & Johnson Pharmaceutical Research & Development LLC, San Diego, CA 92121, USA. pbonave1@prdus.jnj.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry / genetics* Cluster Analysis Gene Expression / physiology Gene Expression Profiling / methods, statistics & numerical data Genetic Markers Hippocampus / chemistry, metabolism* In Situ Hybridization Male Mesencephalon / chemistry, metabolism* Oligonucleotide Array Sequence Analysis / methods, statistics & numerical data Paraventricular Hypothalamic Nucleus / chemistry, metabolism* Polymerase Chain Reaction / methods RNA, Messenger / analysis, biosynthesis Rats Rats, Wistar Receptor, Serotonin, 5-HT2B Receptors, Serotonin / biosynthesis, genetics |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers; 0/RNA, Messenger; 0/Receptor, Serotonin, 5-HT2B; 0/Receptors, Serotonin |
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