Document Detail

Nucleases isolated from Chelidonium majus L. milky sap can induce apoptosis in human cervical carcinoma HeLa cells but not in Chinese Hamster Ovary CHO cells.
MedLine Citation:
PMID:  18296268     Owner:  NLM     Status:  MEDLINE    
Milky sap isolated from Chelidonium majus L. (Greater Celandine) serves as a rich source of various biologically active substances such as alkaloids, flavonoids and phenolic acids. Previous research showed that the activity of Ch. majus milky sap may depend also on the presence of biologically active proteins. The goal of this study was to evaluate the biological effect of two nucleases isolated from Ch. majus milk sap, CMN1 of 20 kDa and CMN2 of 36 kDa, on HeLa and CHO tumour cell lines. Both studied nucleases together with other proteins in the sap of the plant are involved in stress and defence reactions against different pathogens. After 48 h incubation of CMN1 and CMN2 only with HeLa cells, the dependence between the number of apoptotic lesions and the concentration of applied nuclease was observed. The highest proapoptotic activity was induced by 13.3 ng/ml concentration of CMN2 collected in May (62 +/- 3% HeLa cells were apoptotic). Moreover, the proportion of necrotic cells in all concentrations of the nucleases and both cell lines was relatively low (1-8 +/- 0.5%). In summary, results of this study show that purified nucleases CMN1 and CMN2 isolated from Ch. majus milky sap exhibit apoptotic activity in HeLa tumour cell line, but not in CHO cells, without inflammatory reaction.
Robert Nawrot; Maria Wołuń-Cholewa; Anna Goździcka-Józefiak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  46     ISSN:  1897-5631     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-02-25     Completed Date:  2008-04-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  79-83     Citation Subset:  IM    
Department of Molecular Virology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
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MeSH Terms
Apoptosis / drug effects*
CHO Cells
Chelidonium / enzymology*
Chemical Fractionation
Deoxyribonucleases / isolation & purification*,  pharmacology*
Hela Cells
Plant Extracts / pharmacology*
Uterine Cervical Neoplasms / pathology*
Reg. No./Substance:
0/Plant Extracts; EC 3.1.-/Deoxyribonucleases

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