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Nucleases in Bdellovibrio bacteriovorus contribute towards efficient self-biofilm formation and eradication of pre-formed prey biofilms.
MedLine Citation:
PMID:  23297829     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Bdellovibrio bacteriovorus are predatory bacteria that burrow into prey bacteria and degrade their cell contents, including DNA and RNA, to use for growth. Their genome encodes diverse nucleases, some with potential export sequences. Transcriptomic analysis determined two candidate predicted nuclease genes (bd1244, bd1934) upregulated upon contact with prey; which, we hypothesised, may be involved in prey nucleic acid degradation. RT-PCR on total RNA from across the predatory cycle confirmed that the transctription of these genes peaks shortly after prey cell invasion, around the time that prey DNA is being degraded. We deleted bd1244 and bd1934 both singly and together and investigated their role in predation of prey cells and biofilms. Surprisingly, we found that the nuclease mutant strains could still prey upon planktonic bacteria as efficiently as wild-type and still degraded the prey genomic DNA. The Bdellovibrio nuclease mutants were less efficient at (self-) biofilm formation and surprisingly they showed enhanced predatory-clearance of preformed prey cell biofilms relative to wild-type Bdellovibrio © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Carey Lambert; R Sockett
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Publication Detail:
Type:  LETTER     Date:  2013-1-9
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
University of Nottingham.
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