Document Detail

Nuclear uptake of monoclonal antibody to a surface glycoprotein and its effect on transcription.
MedLine Citation:
PMID:  2658809     Owner:  NLM     Status:  MEDLINE    
Nuclear transport and chromatin binding of monoclonal antibody (MAb) ME491, directed against a cell surface glycoprotein, was tested in intact cells and in a cell-free system. After a 24-h incubation with 125I-MAb ME491, the chromatin of melanoma cells and of colorectal carcinoma cells contained approximately 10 and 20%, respectively, of the antibody in nondegraded form. 125I-MAb ME491 was bound to a 55-kDa chromatin protein and localized in two HincII-digested chromatin fragments. Taken up by the nucleus, MAb ME491 inhibited transcription of ribosomal RNA genes by 70%. Nuclear uptake of 125I-MAb ME491 was increased up to ninefold when cells were preincubated with puromycin or actinomycin D. Nuclear uptake of MAb ME491 in a cell-free system was inhibited by ME491 antigen newly synthesized in the cytoplasm. Binding of 125I-MAb ME491 to the newly synthesized ME491 antigen caused precipitation of polysome-bound ME491 mRNA. The effect of MAb ME491 on transcription is discussed.
E M Rakowicz-Szulczynska; H Koprowski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  271     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-07-03     Completed Date:  1989-07-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  366-79     Citation Subset:  IM    
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
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MeSH Terms
Antibodies, Monoclonal* / analysis
Antibody Specificity
Antigens, Neoplasm / analysis,  genetics,  immunology*
Biological Transport
Cell Nucleus / immunology*
Cell-Free System
Chromatin / immunology
Colorectal Neoplasms / immunology
Cytoplasm / immunology
Deoxyribonuclease BamHI
Deoxyribonucleases, Type II Site-Specific
Fluorescent Antibody Technique
Melanoma / immunology
Membrane Glycoproteins / genetics,  immunology*
Nucleic Acid Hybridization
RNA, Messenger / analysis
RNA, Ribosomal / genetics
Receptor, Epidermal Growth Factor / immunology
Transcription, Genetic*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Chromatin; 0/Membrane Glycoproteins; 0/RNA, Messenger; 0/RNA, Ribosomal; EC, Epidermal Growth Factor; EC 3.1.21.-/Deoxyribonuclease BamHI; EC, Type II Site-Specific; EC type II deoxyribonucleases

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